Nucleotide sequence of the VP4 core protein gene (M4 RNA) of US bluetongue virus serotype 10

Yu, Yado; Fukusho, Akio; Roy, Polly

doi:10.1093/nar/15.17.7206

Cited by 20 publications

(7 citation statements)

References 4 publications

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“…The amino terminus of VP7 can tolerate additions when involved in the formation of CLPs. The amino terminus of VP7 is generally hydrophilic (19,27). To investigate whether the amino terminus of the protein can tolerate additional sequences, an extension mutant with an additional 11-aminoacid rabies virus sequence (VP7 N-E [ Fig.…”

Section: Resultsmentioning

confidence: 99%

A single point mutation in the VP7 major core protein of bluetongue virus prevents the formation of core-like particles

Blois

Roy

1993

J Virol

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To understand the assembly process of bluetongue virus (BTV), we have established a functional assay which allows us to produce and manipulate BTV core-like particles (CLPs) composed of the viral VP7 and VP3 proteins. A cDNA clone encoding the 349-amino-acid VP7 protein has been manipulated to generate deletion, extension, and site-specific mutants. Each mutant was coexpressed with the BTV VP3 protein to generate CLPs. Deletion and extension mutants involving the VP7 carboxy terminus prevented CLP formation, while an extension mutant involving an 11-amino-acid rabies virus sequence added to the amino terminus of VP7 allowed CLP formation. Substitution of either of two cysteine residues ofVP7 (Cys-15 or Cys-65) by serine also did not prevent CLP formation; however, substitution of the single lysine residue of VP7 (Lys-255) by leucine abrogated CLP formation, indicating a critical role for this lysine.

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Section: Resultsmentioning

confidence: 99%

A single point mutation in the VP7 major core protein of bluetongue virus prevents the formation of core-like particles

Blois

Roy

1993

J Virol

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“…4, amino acid 255; Yu et al, 1987;Kowalik et al, 1990). In the EHDV VP7 sequence there is also a lysine residue at that position.…”

Section: Properties Of the Ehd V-1 $7 Rna-encoded Product The Vp7 Prmentioning

confidence: 99%

Characterization of the genes encoding two of the major capsid proteins of epizootic haemorrhagic disease virus indicates a close genetic relationship to bluetongue virus

Iwata

Chuma

Roy

1992

Journal of General Virology

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The sequences of the genes of two of the major capsid proteins of epizootic haemorrhagic disease virus serotype 1 (EHDV-1, Orbivirus genus, Reoviridae) have been determined by analyses of cDNA clones representing the L2 and $7 RNA segments. The EHDV-1 $7 RNA segment, which encodes the VP7 core protein, is 1162 nucleotides in length and has the capacity to encode 349 amino acids (Mr 38243). The EHDV-1 L2 RNA segment, which encodes the outer capsid VP2 protein (Mr 113 249) is 2968 nucleotides in length and has an open reading frame of 971 codons. The potential secondary structure of the EHDV-1 $7 mRNA species, in particular that of the terminal regions, is comparable to those of the corresponding segments of bluetongue virus (BTV) and African horse sickness virus (AHSV); the EHDV-1 L2 mRNA species has a secondary structure similar to that of the L2 mRNA of BTV. The EHDV-1 VP2 and VP7 proteins, as well as those of the other two major structural proteins of EHDV published previously (the inner core VP3 protein and the second outer capsid, VP5), are closely related to the corresponding proteins of BTV. The EHDV and BTV VP7 sequences are more distantly related to the sequence of the AHSV VP7 protein published recently.

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“…Towards this end we have sequenced cDNA clones of the genomic RNA species. Eight of the 10 dsRNA segments of BTV-10 have already been cloned and sequenced (Purdy et al, , 1986Ghiasi et al, 1985 ;Lee & Roy, 1986, 1987Yu et al, 1987Yu et al, , 1988Roy et al, 1988). In this paper we complete the sequence analyses by describing the molecular cloning and nucleotide sequence of the remaining two small RNA segments, $8 and $9.…”

Section: Introductionmentioning

confidence: 99%

Completion of the Sequence of Bluetongue Virus Serotype 10 by the Characterization of a Structural Protein, VP6, and a Non-structural Protein, NS2

Fukusho

Yamaguchi

et al. 1989

Journal of General Virology

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Nucleotide sequence of the VP4 core protein gene (M4 RNA) of US bluetongue virus serotype 10

Cited by 20 publications

References 4 publications

A single point mutation in the VP7 major core protein of bluetongue virus prevents the formation of core-like particles

A single point mutation in the VP7 major core protein of bluetongue virus prevents the formation of core-like particles

Characterization of the genes encoding two of the major capsid proteins of epizootic haemorrhagic disease virus indicates a close genetic relationship to bluetongue virus

Completion of the Sequence of Bluetongue Virus Serotype 10 by the Characterization of a Structural Protein, VP6, and a Non-structural Protein, NS2

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