Nucleotide sequence variants of Rattus norvegicus mitochondrial DNA

Goddard, Judy M.; Masters, Jeffrey N.; Jones, Suzan S.; Ashworth, William D.; Wolstenholme, David R.

doi:10.1007/bf00285770

Cited by 17 publications

(7 citation statements)

References 24 publications

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“…This is particularly significant for the HTC hepatoma, since this is an established rat hepatoma cell line reported by Corral et al (1989) to possess a recombinant form of COI and ND6. These results confirm and extend a previous report by Goddard et al (1981) that mtDNA from the Novikoff and Chang rat hepatomas showed restriction patterns identical to those of control variant A rat mtDNA. Both Novikoff and Chang hepatomas were derived from the Sprague-Dawley strain of Rattus norvegicus.…”

Section: Discussionsupporting

confidence: 94%

“…Although the basis for the apparent discrepancy between our results and those of Corral et al (1989) is not known, it should be noted that at least two variants of rat mtDNA with different restriction endonuclease cleavage patterns have been described (Francisco et al, 1979;Goddard et al, 1981). All of our results on hepatoma and normal rat mtDNA, as well as the results reported by Corral et al (1989) for hepatoma mtDNA, are consistent with the variant A rat mtDNA sequence and restriction pattern (Francisco et al, 1979;Gadaleta et al, 1989).…”

Section: Discussionsupporting

confidence: 83%

“…4). In all of these experiments, the apparent sizes of the mtDNA fragments matched quite well with the published mtDNA sequence (Gadaleta et al, 1989) and with previous reports on restriction fragment sizes for rat mtDNA (Saccone et aL, 1976;Francisco et al, 1979;Goddard et al, 1981). We conclude that since there seems to be relatively little polymorphism in the rat mitochondrial genomes of tumor and normal tissues, it appears unlikely that large-scale changes in mtDNA sequence play a significant role in carcinogenesis.…”

Section: Discussionsupporting

confidence: 79%

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Restriction fragment length polymorphism (RFLP) analysis provides evidence for a high degree of homology of mitochondrial DNAs from rat hepatomasVersus normal rat livers

Nakashima

Bayouth

et al. 1992

Biochem Genet

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Where differences have been reported between tumor and normal mitochondrial DNA (mtDNA), they have generally involved limited modifications of the genome (Taira et al., Nucleic Acids Res. 11:1635, 1983; Shay and Werbin, Mutat. Res. 186:149, 1987). However, Corral et al. (Nucleic Acids Res. 16:10935, 1988; 17:5191, 1989) observed recombination between cytochrome oxidase subunit I (COI) and NADH dehydrogenase subunit 6 (ND6), two genes normally on opposite sides of the circular mitochondrial genome. In rat hepatoma mtDNA COI and ND6 were reported to be separated by only 230 base pairs (Corral et al., 1988, 1989). We have performed RFLP analysis on mtDNA from normal rat livers and rat hepatomas, using COI and ND6 probes. Additional experiments compared end-labeled DNA fragments produced by EcoRI and HindIII digestion of mtDNA. These studies failed to provide any evidence for genetic recombination in rat hepatoma mtDNA, even in the same cell line used by Corral et al. Rather, they support the conclusion that mtDNA from tumor and normal tissues exhibits a low degree of heterogeneity.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionsupporting

confidence: 83%

Section: Discussionsupporting

confidence: 79%

See 1 more Smart Citation

Restriction fragment length polymorphism (RFLP) analysis provides evidence for a high degree of homology of mitochondrial DNAs from rat hepatomasVersus normal rat livers

Nakashima

Bayouth

et al. 1992

Biochem Genet

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“…The solid circles are for interspecific comparisons of the segment of mtDNA we sequenced. The empty triangle is based on intraspecific comparisons of two types: (1) The human segment (896 bp) we sequenced and (2) the rat sequence of t69 bp determined by Castora et al (1980), De Vos et al (1980), and Goddard et al (1981). In both curves A and B each point is the average for all comparisons made for a particular divergence time.…”

Section: Predominance Of Transitionsmentioning

confidence: 99%

Mitochondrial DNA sequences of primates: Tempo and mode of evolution

et al. 1982

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We cloned and sequenced a segment of mitochondrial DNA from human, chimpanzee, gorilla, orangutan, and gibbon. This segment is 896 bp in length, contains the genes for three transfer RNAs and parts of two proteins, and is homologous in all 5 primates. The 5 sequences differ from one another by base substitutions at 283 positions and by a deletion of one base pair. The sequence differences range from 9 to 19% among species, in agreement with estimates from cleavage map comparisons, thus confirming that the rate of mtDNA evolution in primates is 5 to 10 times higher than in nuclear DNA. The most striking new finding to emerge from these comparisons is that transitions greatly outnumber transversions. Ninety-two percent of the differences among the most closely related species (human, chimpanzee, and gorilla) are transitions. For pairs of species with longer divergence times, the observed percentage of transitions falls until, in the case of comparisons between primates and non-primates, it reaches a value of 45. The time dependence is probably due to obliteration of the record of transitions by multiple substitutions at the same nucleotide site. This finding illustrates the importance of choosing closely related species for analysis of evolutionary process. The remarkable bias toward transitions in mtDNA evolution necessitates the revision of equations that correct for multiple substitutions at the same site. With revised equations, we calculated the incidence of silent and replacement substitutions in the two protein-coding genes. The silent substitution rate is 4 to 6 times higher than the replacement rate, indicating strong functional constraints at replacement sites. Moreover, the silent rate for these two genes is about 10% per million years, a value 10 times higher than the silent rate for the nuclear genes studied so far. In addition, the mean substitution rate in the three mitochondrial tRNA genes is at least 100 times higher than in nuclear tRNA genes. Finally, genealogical analysis of the sequence differences supports the view that the human lineage branched off only slightly before the gorilla and chimpanzee lineages diverged and strengthens the hypothesis that humans are more related to gorillas and chimpanzees than is the orangutan.

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“…Rapid intraspecific variation in mitochondrial genotypes has been noted for a number of mammalian species (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20). In the majority of these studies, variation was detected by restriction site polymorphism and was explicable by simple gains or losses in sites.…”

mentioning

confidence: 99%