Nucleotide Sequences of Genes Coding for Fimbrial Proteins in a Cryptic Genospecies of
            <i>Haemophilus</i>
            spp. Isolated from Neonatal and Genital Tract Infections

Gousset, Nathalie; Rosenau, Agnès; Sizaret, P; Quentin, Roland

doi:10.1128/iai.67.1.8-15.1999

Cited by 13 publications

(2 citation statements)

References 44 publications

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“…Members of this species comprise part of the bacterial population identified as H. influenzae biotype IV in biochemical tests and were previously referred to as "a cryptic genospecies of Haemophilus" (2,11,12). They form a homogenous group with unique multilocus enzyme electrophoresis patterns, outer membrane protein profiles, and fimbrial protein gene sequences (2,9). In 16S rRNA sequence analysis, they form a monophyletic unit with a closer relation to Haemophilus haemolyticus than to H. influenzae (12).…”

Section: H Quentini Is a New Species Recently Proposed For A Group Ofmentioning

confidence: 99%

Reduced Levofloxacin Susceptibility and Tetracycline Resistance in a Clinical Isolate of Haemophilus quentini Identified by 16S rRNA Sequencing

Mak

Tse

et al. 2005

J Clin Microbiol

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This paper reports the first case of Haemophilus quentini bacteremia with reduced susceptibility to levofloxacin and resistance to nalidixic acid identified by 16S rRNA sequencing. There was an S84L substitution in gyrA and an S84I substitution in parC. The isolate had coresistance to ampicillin (␤-lactamase positive) and tetracycline mediated by the tet(B) gene. CASE REPORTThe patient was a newborn, full-term baby girl. She was delivered by the normal vaginal route with a birth weight of 2.9 kg in a general hospital in Hong Kong. The antenatal history was uncomplicated. There was no history of prolonged rupture of membrane or maternal fever. The baby had an Apgar score of 9 at 1 min. Twelve hours after birth, she developed respiratory distress with insucking of the chest and was admitted to a neonatal intensive care unit. Aspiration from a nasogastric tube revealed a large amount meconium-stained fluid. A chest X ray showed a hyperinflated chest and hazziness over both lung fields. Her total leukocyte count was 34.2 ϫ 10 9 /liter (91% neutrophils). The platelet counts and hemoglobin level were normal. A blood culture was performed. She was resuscitated and empirical intravenous ampicillin and netromycin were commenced. She was put on nasal continuous positive airway pressure for respiratory support. Subsequently, the antimicrobial therapy was changed to intravenous amoxicillin-clavulanic acid and was continued for 7 days. The patient recovered after treatment and was discharged on day 13.Two days later (1 October 2001), the blood culture bottles were positive for a gram-negative coccobacilli. The bacterium (strain S32F2) was grown on chocolate agar to give colonies of 1 mm in diameter after incubation for 24 h at 37°C in air with 5% CO 2 , but there was no growth on blood agar under the same incubation conditions. Results of the identification by biochemical methods are shown in Table 1. The API NH system (bioMerieux Vitek) but not the Vitek NHI system (bioMerieux Vitek) gave Haemophilus influenzae as the identification at a high confidence level. The organism was subsequently characterized by PCR and near complete sequencing of the 16S rRNA gene, using primers and a protocol we previously described (5). In brief, the sequences of the PCR products were compared with known 16S rRNA gene sequences in the GenBank database by BLAST searches. The length of the 16S

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Section: H Quentini Is a New Species Recently Proposed For A Group Ofmentioning

confidence: 99%

Reduced Levofloxacin Susceptibility and Tetracycline Resistance in a Clinical Isolate of Haemophilus quentini Identified by 16S rRNA Sequencing

Mak

Tse

et al. 2005

J Clin Microbiol

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“…More recently, a number of reports have described H. quentini strains isolated from the urinary tracts of adult men and noted their association with urethritis in this group (16,17). Several groups have explored the role of particular adhesins that may allow for enhanced adherence of H. quentini in the urogenital niche (14,(18)(19)(20)(21)(22).…”

mentioning

confidence: 99%

Identification and Characterization of “ Haemophilus quentini ” Strains Causing Invasive Disease in Ontario, Canada (2016 to 2018)

et al. 2019

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Haemophilus influenzae is a well-established human pathogen capable of causing a range of respiratory and invasive diseases. Since the 1970s, it has been observed that a nontypeable cryptic genospecies of H. influenzae, most often biotype IV, has been associated with the genitourinary tracts of females and with invasive neonatal infections. This distinct genospecies has been provisionally named “Haemophilus quentini.” Here, we report seven cases of invasive H. quentini disease in patients from Ontario, Canada, over a 2-year period. Significantly, while most reports of invasive disease with H. quentini to date have been in neonates, we observed five cases in adults (three in women of childbearing age and two in seniors) as well as two in neonates. Identification of H. quentini is challenging and was not possible for frontline laboratories, requiring work at the reference laboratory level. We describe in detail the biochemical results, matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-Tof MS) results, and PCR results with several targets, including the 16S rRNA gene and multilocus sequence typing (MLST) genes, for the seven Ontario H. quentini isolates and several controls. Our data, combined with those of other publications, support the fact that H. quentini is distinct from H. influenzae and Haemophilus haemolyticus. This organism is recognized as a pathogen of neonates, but we hypothesize that it may be underrecognized as an important pathogen in adults as well, particularly pregnant women. By sharing the detailed descriptions of these isolates, we hope to enable other laboratories to better identify H. quentini so that the true prevalence of this organism and disease can be explored.

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Low Sensitivity of Listeria monocytogenes to Quaternary Ammonium Compounds

Mereghetti¹,

Quentin

Mee³

et al. 2000

Appl Environ Microbiol

136

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Ninety-seven epidemiologically unrelated strains of Listeria monocytogenes were investigated for their sensitivities to quaternary ammonium compounds (benzalkonium chloride and cetrimide). The MICs for seven serogroup 1/2 strains were high. Three came from the environment and four came from food; none were isolated from human or animal samples. All 97 strains carried the mdrL gene, which encodes a multidrug efflux pump, and the orfA gene, a putative transcriptional repressor of mdrL. The absence of plasmids in four of the seven resistant strains and the conservation of resistance after plasmid curing suggested that the resistance genes are not plasmid borne. Moreover, PCR amplification and Southern blot hybridization experiments failed to find genes phylogenetically related to the qacA and smr genes, encoding multidrug efflux systems previously described for the genus Staphylococcus. The high association between nontypeability by phages and the loss of sensitivity to quaternary ammonium compounds are suggestive of an intrinsic resistance due to modifications in the cell wall.

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Nucleotide Sequences of Genes Coding for Fimbrial Proteins in a Cryptic Genospecies of Haemophilus spp. Isolated from Neonatal and Genital Tract Infections

Cited by 13 publications

References 44 publications

Reduced Levofloxacin Susceptibility and Tetracycline Resistance in a Clinical Isolate of Haemophilus quentini Identified by 16S rRNA Sequencing

Reduced Levofloxacin Susceptibility and Tetracycline Resistance in a Clinical Isolate of Haemophilus quentini Identified by 16S rRNA Sequencing

Identification and Characterization of “ Haemophilus quentini ” Strains Causing Invasive Disease in Ontario, Canada (2016 to 2018)

Low Sensitivity of Listeria monocytogenes to Quaternary Ammonium Compounds

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