Nucleotide Sequences of the Coat Protein Genes and Flanking Regions of Cucumber Mosaic Virus Strains C and WL RNA 3

Abstract

SUMMARYSeveral strains of cucumber mosaic virus (CMV) have been classified, and nucleic acid hybridization data indicate that these strains differ widely in nucleotide sequence. We have constructed cDN A clones of the coat protein coding regions of CMV strains C and WL, and have compared the nucleotide sequences of the RNA 3 intergenic region, coat protein gene, and 3' untranslated region with published CMV sequences from the same regions of the Q, D and Y strains. These comparisons show that the C and WL strains belong to different CMV subgroups, and that the subgroups are more closely related in sequence than suggested by previous nucleic acid hybridization studies.

Differentiation of cucumber mosaic virus isolates using the polymerase chain reaction

“…Isolates in MspI restriction group I produced a single D N A product and isolates in restriction group 2 produced the same product and an additional minor product. It is worth noting that the possible priming sites Q 248, 197, 158, 126, 110, 28 S Davies & Symons (1988) WL 248, 197, 158, 126, I10, 28 S Quemada et al (1989 MspI group 1 This work CP91/454 248, 197, 158, 126, 110, Comparison of the c D N A sequences from some of our isolates with those published for six other CMV isolates (Davies & Symons, 1988;Quemada et al, 1989;Hayakawa et al, 1989;Owen et al, 1990) confirmed that only our two MspI restriction patterns were present. Furthermore our restriction groups divided the six other CMV isolates into their previously established subgroups (Table 2).…”

Biological and Molecular Characterization of Cucumber mosaic virus Subgroup II Isolate Causing Severe Mosaic in Cucumber

“…While coat protein is required for encapsidation of the genomic RNAs into virus particles, important in aphid transmission [7,30] and affecting symptom expression [42,49]. CMV strains are divided into two subgroups I and II [3,31,33] and subgroup I is subdivided into IA and IB based on nucleotide sequence of the 3 0 untranslated region of RNA3 [37]. Nucleotide sequence similarity among IA and IB subgroup strains is 92-94 %.…”

Coat protein gene sequences of two cucumber mosaic virus strains reveal a single amino acid change correlating with chlorosis induction

“…The putative coat protein amino acid sequences of these strains were compared with those of other chlorosis-inducing (M-and Y-CMV) as well as mosaic-inducing (Fny-, O-, D-and C-CMV) strains (Owen et al, 1990;Hayakawa et al, 1988Hayakawa et al, , 1989Cuozzo et al, 1988;Quemada et al, 1989).…”