Nucleotides adjacent to N6-methyladenosine in maize poly(A)-containing RNA

Nichols, J. L.; Welder, L.

doi:10.1016/0304-4211(81)90071-7

Cited by 46 publications

(45 citation statements)

References 18 publications

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“…In both plant and mammal mRNA, m 6 A occurs only within sequences matching the consensus GAC or AAC (Wei et al, 1976;Nichols and Welder, 1981). It has been demonstrated that there is a preferred five-nucleotide consensus sequence, RRACH (Schibler et al, 1977).…”

Section: Mta Interaction With At Fip37 and Possible Functions Of M 6 mentioning

confidence: 99%

“…Ribonuclease fragmentation and labeling studies on mRNA from animal cells and maize tissues show m 6 A to be present only at the central A within the defined sequence context GAC and AAC, with a 75% preference for GAC (Wei et al, 1976;Schibler et al, 1977;Nichols and Welder, 1981;Harper et al, 1990).…”

Section: Introductionmentioning

confidence: 99%

“…N 6 -Methyladenosine (m 6 A) is found in the mRNA of some viruses (Beemon and Keith, 1977;Aloni et al, 1979) and all multicellular eukaryotes examined, including mammals (Adams and Cory, 1975;Perry et al, 1975;Wei et al, 1976), insects (Levis and Penman, 1978), and the monocot plants maize (Zea mays; Nichols, 1979), wheat (Triticum aestivum; Kennedy and Lane, 1979), and oat (Avena sativa; Haugland and Cline, 1980). Ribonuclease fragmentation and labeling studies on mRNA from animal cells and maize tissues show m 6 A to be present only at the central A within the defined sequence context GAC and AAC, with a 75% preference for GAC (Wei et al, 1976;Schibler et al, 1977;Nichols and Welder, 1981;Harper et al, 1990).…”

Section: Introductionmentioning

confidence: 99%

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MTA Is an Arabidopsis Messenger RNA Adenosine Methylase and Interacts with a Homolog of a Sex-Specific Splicing Factor

et al. 2008

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N 6 -Methyladenosine is a ubiquitous modification identified in the mRNA of numerous eukaryotes, where it is present within both coding and noncoding regions. However, this base modification does not alter the coding capacity, and its biological significance remains unclear. We show that Arabidopsis thaliana mRNA contains N 6 -methyladenosine at levels similar to those previously reported for animal cells. We further show that inactivation of the Arabidopsis ortholog of the yeast and human mRNA adenosine methylase (MTA) results in failure of the developing embryo to progress past the globular stage. We also demonstrate that the arrested seeds are deficient in mRNAs containing N 6 -methyladenosine. Expression of MTA is strongly associated with dividing tissues, particularly reproductive organs, shoot meristems, and emerging lateral roots. Finally, we show that MTA interacts in vitro and in vivo with At FIP37, a homolog of the Drosophila protein FEMALE LETHAL2D and of human WILMS' TUMOUR1-ASSOCIATING PROTEIN. The results reported here provide direct evidence for an essential function for N 6 -methyladenosine in a multicellular eukaryote, and the interaction with At FIP37 suggests possible RNA processing events that might be regulated or altered by this base modification.

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Section: Mta Interaction With At Fip37 and Possible Functions Of M 6 mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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MTA Is an Arabidopsis Messenger RNA Adenosine Methylase and Interacts with a Homolog of a Sex-Specific Splicing Factor

et al. 2008

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“…This protein is required for the splicing regulation of Sxl (Sex lethal) and tra (transformer) pre-mRNAs, two critical gene transcripts associated with Drosophila sex determination and dosage compensation (Penalva et al 2000;Ortega et al 2003;Penn et al 2008). m 6 A methylation in plants m 6 A is a ubiquitous modification found in the mRNAs of various plants, including monocot plants maize (Nichols 1979), wheat (Kennedy and Lane 1979), oat (Haugland and Cline 1980), A. thaliana (Zhong et al 2008;Luo et al 2014), and rice (Li et al 2014). MTA (encoded by At4g10760), a METTL3 ortholog in Arabidopsis, has been identified as an active component of the m 6 A methyltransferase complex (Zhong et al 2008).…”

Section: Conservation Of M 6 a Rna Methylation Machinery And Its Relamentioning

confidence: 99%

RNA N⁶-methyladenosine methylation in post-transcriptional gene expression regulation

2015

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N6 -methyladenosine (m 6 A) is the most prevalent and internal modification that occurs in the messenger RNAs (mRNA) of most eukaryotes, although its functional relevance remained a mystery for decades. This modification is installed by the m 6 A methylation "writers" and can be reversed by demethylases that serve as "erasers." In this review, we mainly summarize recent progress in the study of the m 6 A mRNA methylation machineries across eukaryotes and discuss their newly uncovered biological functions. The broad roles of m 6 A in regulating cell fates and embryonic development highlight the existence of another layer of epigenetic regulation at the RNA level, where mRNA is subjected to chemical modifications that affect protein expression.

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“…In addition to splicing, influenza virus mRNAs have been shown to contain internal N6-methyladenosine (m6A) residues. m6A is a common posttranscriptional modification found in cellular and viral mRNAs synthesized in the nucleus (5,12) at the consensus sequences Gm6AC or Am6AC (14,18). Evidence derived from localization (6, 7) and inhibitor (3) studies suggests that this modification may play a role in mRNA metabolism.…”

mentioning

confidence: 99%

Unequal distribution of N6-methyladenosine in influenza virus mRNAs.

Narayan¹,

Ayers²,

Rottman³

et al. 1987

Mol. Cell. Biol.

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Influenza virus mRNA is posttranscriptionally methylated at internal adenosine residues to form N6-methyladenosine (m6A). It has been previously shown that there is an average of three m6A residues per influenza virus mRNA (R. M. Krug, M. A. Morgan, and A. J. Shatkin, J. Virol. 20:45-53, 1976). To determine the distribution of m6A in the different influenza virus mRNAs, we purffied six of the mRNAs by hybrid selection, digested them with nuclease, and determined their methylation patterns by high-pressure liquid chromatography. The amount of m6A in the different mRNAs varied from one in matrix to eight in hemagglutinin.Influenza virus has a negative-strand RNA genome composed of eight gene segments (11). With the exception of retroviruses, influenza virus is the only RNA virus which replicates in the nuclei of infected cells. Nuclear replication allows influenza virus to utilize cell functions involved in the processing of nuclear RNA precursors. In this regard, two influenza virus mRNAs, M and NS, coding for the matrix protein and nonstructural protein, respectively, are themselves spliced to form smaller mRNAs, coding for two other proteins, M2 and NS2, respectively (11). In addition to splicing, influenza virus mRNAs have been shown to contain internal N6-methyladenosine (m6A) residues. m6A is a common posttranscriptional modification found in cellular and viral mRNAs synthesized in the nucleus (5, 12) at the consensus sequences Gm6AC or Am6AC (14,18). Evidence derived from localization (6, 7) and inhibitor (3) studies suggests that this modification may play a role in mRNA metabolism.It has previously been shown that influenza virus mRNA contains an average of three m6A residues per molecule, one of which may be derived from the 5' end of the host cell mRNA used to prime viral transcription (10). To study the possible significance of m6A in influenza virus mRNA metabolism, we determined the distribution of m6A residues in six individual hybrid-selected mRNAs.

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Nucleotides adjacent to N6-methyladenosine in maize poly(A)-containing RNA

Cited by 46 publications

References 18 publications

MTA Is an Arabidopsis Messenger RNA Adenosine Methylase and Interacts with a Homolog of a Sex-Specific Splicing Factor

MTA Is an Arabidopsis Messenger RNA Adenosine Methylase and Interacts with a Homolog of a Sex-Specific Splicing Factor

RNA N⁶-methyladenosine methylation in post-transcriptional gene expression regulation

Unequal distribution of N6-methyladenosine in influenza virus mRNAs.

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Nucleotides adjacent to N6-methyladenosine in maize poly(A)-containing RNA

Cited by 46 publications

References 18 publications

MTA Is an Arabidopsis Messenger RNA Adenosine Methylase and Interacts with a Homolog of a Sex-Specific Splicing Factor

MTA Is an Arabidopsis Messenger RNA Adenosine Methylase and Interacts with a Homolog of a Sex-Specific Splicing Factor

RNA N6-methyladenosine methylation in post-transcriptional gene expression regulation

Unequal distribution of N6-methyladenosine in influenza virus mRNAs.

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Product

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RNA N⁶-methyladenosine methylation in post-transcriptional gene expression regulation