Nuleotide sequence of the celA gene encoding a cellodextrinase of Ruminococcus flavefaciens FD-1

Wang, Wenyen; Thomson, Jennifer A.

doi:10.1007/bf00265451

Cited by 18 publications

(23 citation statements)

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“…Of the 6000 clones tested, nine had CMCase activity but none had activity on MUC. Preliminary restriction enzyme maps and xylanase assays showed that five of the inserts carried the ceZA gene previously cloned on pMEB200 (Wang & Thomson, 1990), three carried the same 3.6 kb insert and had xylanase activity and one carried a different insert PstI-PuuI fragment used as a probe in RNA dot-blot analysis. P,, lambda P, promoter.…”

Section: Resultsmentioning

confidence: 99%

“…The cell debris was removed by centrifugation for 15 min at 27000 g and the extract stored at -20 "C. Cellulase and xylanase activities were determined by the 3,5-dinitrosalicylic acid (DNS) method (Miller, 1959) using CMC (0-5 YO, w/v) or xylan (0.25 YO, w/v) as substrates. Activity on p-nitrophenyl P-D-cellobioside @NPC) was as described previously (Wang & Thomson, 1990).…”

Section: Methodsmentioning

confidence: 99%

“…1). The cellodextrinase gene probe was a 1-2 kb HindIII fragment of pSKl (Wang & Thomson, 1990). The probes were labelled with [32P]dCTP using a nick-translation kit (Amersham) and lo6 d.p.m.…”

Section: Methodsmentioning

confidence: 99%

“…We were interested to investigate whether cellobiose and cellotriose, the main products of cellulose degradation by cellulases of R. Jlavefaciens FD-1 (Rasmussen et al, 1988), affected the transcription of cellulase genes. We therefore cloned a gene which encoded an enzyme with CMCase and xylanase activity and used it, together with a previously cloned cellodextrinase gene (Wang & Thomson, 1990), as probes to investigate RNA levels in cells grown on cellobiose, cellotriose and cellulose. Our choice of cellobiose was based on the findings that R.JEavefaciens appears to lack an extracellular P-glucosidase, is unable to metabolize extracellular glucose, but grows well on cellobiose (Rasmussen et al, 1988).…”

Section: Introductionmentioning

confidence: 99%

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Transcriptional regulation of an endoglucanase and a cellodextrinase gene in Ruminococcus flavefaciens FD-1

Wang

Reid²,

Thomson³

1993

Journal of General Microbiology

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A gene which encodes a 35 kDa protein with both carboxymethylcellulase and xylanase activity was cloned from RuminococcusJEavefaciens FD-1 and the nucleotide sequence determined. The FD-1 gene, cell?, and the ceZA gene, which encodes a cellodextrinase, were used as probes to analyse transcription in R. flavefaciens grown under different conditions. Transcription of both genes was induced when cellulose was added to cells growing in cellobiose. This induction continued after cellulose depletion and after cell division had ceased. Transcription of both genes was also induced by cellotriose, although the effect was not as pronounced as induction by cellulose and was greater for the ceZA gene than for the ceZE gene.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Transcriptional regulation of an endoglucanase and a cellodextrinase gene in Ruminococcus flavefaciens FD-1

Wang

Reid²,

Thomson³

1993

Journal of General Microbiology

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“…Homology of the amino acid sequence with those of other enzymes The deduced amino acid sequence of the Endo-K protein was compared with those of enzymes, reported to date, from various strains of Bacillus (Fukumori et al, 1986a (Nakamura et al, 1986;Wong et al, 1986), Ruminococcus (Ohmiya et al, 1989;Poole et al, 1990;Wang & Thomson, 1990), Butyrivibrio (Berger et al, 1989 ;Hazlewood et al, 1990), Pseudomonas , Erwinia (Guiseppi et al, 1988), Streptomyces (Nakai et al, 1988) and Trichoderma (Penttila et al, 1986;Saloheimo et al, 1988). The sequence from amino acids 56 to 443 of Endo-K exhibited 30% homology to that of the celA endoglucanase from Clostridium thermocellum NCIB 10682 (Bkguin et al, 1985).…”

Section: F S D S Y N L L T M L F L T G N W W K P I P D E K K I Q S P mentioning

confidence: 99%

Molecular cloning and nucleotide sequence of the gene encoding an endo-1,4- -glucanase from Bacillus sp. KSM-330

Ozaki

Sumitomo²,

Ito³

1991

Journal of General Microbiology

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The gene encoding an acid endo-1,4-P-glucanase from Bacillus sp. KSM-330 was cloned into the Hind111 site of pBR322 and expressed in Escherichia cofi HB101. The recombinant plasmid contained a 3.1 kb Hind111 insert, 1-8 kb of which was sufficient for the expression of endoglucanase activity in E cofi HB101. Nucleotide sequencing of this region (1816 bp) revealed an open reading frame of 1389 bp. The protein deduced from this sequence was composed of 463 amino acids with an M, of 51 882. The deduced amino acid sequence from amino acids 56 through 75 coincided with the amino-terminal sequence of the endoglucanase, Endo-K, purified from culture of Baciffus sp. KSM-330. The deduced amino acid sequence of Endo-K had 30% homology with that of the cefA enzyme from Cfostridum thermoceffum NCIB 10682 and 25 % homology with that of the enzyme from Ceffufomonas uda CB4.However, the Endo-K protein exhibited no homology with respect to either the nucleotide or the amino acid sequences of other endoglucanases from Buciffus that had been previously characterized. These results indicate that the gene for Endo-K in Bacillus sp. KSM-330 has evolved from an ancestral gene distinct from that of other Bacillus endoglucanases.

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Polysaccharide Degradation in the Rumen and Large Intestine

Forsberg¹,

Cheng²,

White³

1997

Gastrointestinal Microbiology

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Nuleotide sequence of the celA gene encoding a cellodextrinase of Ruminococcus flavefaciens FD-1

Cited by 18 publications

References 1 publication

Transcriptional regulation of an endoglucanase and a cellodextrinase gene in Ruminococcus flavefaciens FD-1

Transcriptional regulation of an endoglucanase and a cellodextrinase gene in Ruminococcus flavefaciens FD-1

Molecular cloning and nucleotide sequence of the gene encoding an endo-1,4- -glucanase from Bacillus sp. KSM-330

Polysaccharide Degradation in the Rumen and Large Intestine

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