Number and distribution of silver‐stained nucleolar organizer regions and evolutionary relationships in domestic pigs

Liu, W S; Lu, Xiaoping; Zhang, Hanqin Qiu

doi:10.1111/j.1365-2052.1995.tb02662.x

Cited by 9 publications

(4 citation statements)

References 12 publications

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“…Cytogenetic studies have shown that NORs are useful tools in many kinds of animal research, from NORs localization and polymorphism to the relationship between breeds and species (Liu et al 1995;Zijlstra et al 1997;Schempp et al 1998;Danielak-Czech et al 1999;Santani et al 2002).…”

Section: Introductionmentioning

confidence: 99%

The mechanisms determining the nucleolar‐organizing regions inactivation of domestic horse chromosomes

Słota

Wnuk

Bugno

et al. 2007

J Animal Breeding Genetics

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Cytogenetic investigations of the nucleolar-organizing regions (NORs) show that there is variation in the transcriptional activity of rDNA in many organisms. As a consequence, genetic polymorphism of these regions has been detected. The aim of the present study was to evaluate the hypothetic genetic mechanisms determining the NORs polymorphism of the domestic horse chromosomes. Molecular cytogenetic analyses were carried out on Hucul horses and the following techniques were used: fluorescence in situ hybridization (FISH), telomere primed in situ synthesis (PRINS), in situ nick-translation with HpaII, silver staining (AgNOR) and C-banding technique (CBG). The obtained results suggest that variation in the number and size of silver deposits is related to the number of rDNA copies, DNA methylation and the localization of ribosomal DNA loci in telomeric regions. Moreover, we have found that chromosome pairs 28 and 31 are characterized by higher variation in the NORs number.

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Section: Introductionmentioning

confidence: 99%

The mechanisms determining the nucleolar‐organizing regions inactivation of domestic horse chromosomes

Słota

Wnuk

Bugno

et al. 2007

J Animal Breeding Genetics

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“…Liu et al . [ 24 ] stated that the polymorphism of Ag-NORs in pigs is caused mainly by differences in frequency of silver deposits on chromosome 8.…”

Section: Resultsmentioning

confidence: 99%

Nucleolar organizer region banding in crossbred and non-descript pigs of India

Harshini¹,

Devi²,

Kumari³

et al. 2018

Vet World

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Aim:The objective of this experiment was to study the nucleolar organizer region (NOR)-banding pattern in Large White Yorkshire (LWY) crossbred and non-descript pigs and finding differences in the number of NORs between animals and between genetic groups.Materials and Methods:The experiment was carried out on 15 females, and 15 males of LWY crossbred and non-descript pigs to study NOR-banding pattern by employing ammoniacal silver staining technique.Results:A total of 63 and 65 number of good metaphases were prepared in LWY crossbred, and non-descript pigs and a total of 168 and 143 number of NORs were detected on the 8th and 10th chromosomes in both genetic groups, respectively. The mean number of NORs per metaphase was 2.67 and 2.20 in LWY crossbred and non-descript pigs, respectively. LWY crossbred pig had high mean number of silver-stained NORs (Ag-NORs) per metaphase compared to non-descript pig. In general, it was observed that the highest frequency of metaphases (%) examined had two number of NORs, while the lowest frequency (%) had four number of NORs. The number of NORs observed per metaphase on secondary constrictions of the 8th and 10th chromosome pair in both genetic groups ranged from 2 to 4. The Chi-square test of significance revealed that the observed frequencies do not differ significantly from the expected frequencies.Conclusion:The results confirmed differences across breeds in occurrence and number of NORs on chromosomes in pigs. The mean numbers of NORs present per metaphase vary between the animals indicating the existence of polymorphism for the number of NORs. A higher number of Ag-NORs were observed on chromosome pair 10 in both the genetic groups. It was concluded that NORs were more morphologically distinct and greater on chromosome pair 10 than on pair 8, which suggests a dominant role of chromosome 10 in the global production of ribosomal RNA.

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“…RH FW map length for a given region is influenced by marker density; the higher the marker density, the longer the map length (cR); thus, the higher the map resolution. A second is the localization of the NORs, or the ribosomal RNA genes (RNR, or rDNA) on SSC10p near the centromere [ 31 - 34 ]. SSC10 is one of two chromosomes (the other one is SSC8) in the porcine genome that harbors ribosomal RNA genes (RNR, or rDNA) in NORs located in the proximal region of SSC10p near the centromere.…”

Section: Resultsmentioning

confidence: 99%

“…SSC10 is one of two chromosomes (the other one is SSC8) in the porcine genome that harbors ribosomal RNA genes (RNR, or rDNA) in NORs located in the proximal region of SSC10p near the centromere. Silver-staining (Ag-NOR) indicates that the porcine RNR genes on SSC10 are constitutively active in all pig breeds, while their activity on SSC8 varies among different pig breeds [ 31 ]. The ratio of FW vs. non-FW markers was 1:4 (3 FW/12 non-FW) in the NOR region (Fig.…”

Section: Resultsmentioning

confidence: 99%

An integrated RH map of porcine chromosome 10

et al. 2009

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Background: Whole genome radiation hybrid (WG-RH) maps serve as "scaffolds" to significantly improve the orientation of small bacterial artificial chromosome (BAC) contigs, order genes within the contigs and assist assembly of a sequence-ready map for virtually any species. Here, we report the construction of a porcine: human comparative map for pig (Sus scrofa) chromosome 10 (SSC10) using the IMNpRH2 12,000-rad porcine WG-RH panel, integrated with the IMpRH 7000-rad WG-RH, genetic and BAC fingerprinted contig (FPC) maps.

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Number and distribution of silver‐stained nucleolar organizer regions and evolutionary relationships in domestic pigs

Cited by 9 publications

References 12 publications

The mechanisms determining the nucleolar‐organizing regions inactivation of domestic horse chromosomes

The mechanisms determining the nucleolar‐organizing regions inactivation of domestic horse chromosomes

Nucleolar organizer region banding in crossbred and non-descript pigs of India

An integrated RH map of porcine chromosome 10

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