Number and volume of rat dorsal root ganglion cells in acrylamide intoxication

Abstract

Acrylamide intoxication induces a filamentous neuropathy with breakdown of distal axons and chromatolytic reaction of dorsal root ganglion cells. To obtain quantitative information about the perikaryal alterations neurons of the fifth lumbar dorsal root ganglion of rats were examined with stereological techniques following intoxication with a total dose of 500 mg acrylamide. Number, mean volume and distribution of neuron volume were estimated for each of the two cell subpopulations using optical disectors, the four-way-nucleator and systematic sampling techniques. In intoxicated rats perikaryal volume of A-cells was significantly reduced by 28%, from 63,200 micron3 (CV = 0.16) to 45,500 micron3 (CV = 0.19), whereas the volume of B-cells was unchanged. Numbers of A- and B-cells were preserved. The finding of a selective atrophy of A-cell perikaryal volume is in accordance with previous observations of predominant alterations of large myelinated sensory fibres and most likely reflects an attack on the perikaryal neurofilaments abundant in this cell type.

Neurotoxicity of Acrylamide in Exposed Workers

“…According to this hypothesis, direct neurotoxic actions at cell body sites caused deficient production and transport of axon-directed components. The dying-back theory was supported by some following studies, suggesting that sensory and motor nerve cell body remodeling observed during intoxication was a direct neuropathogenic effect of ACR [24,25,26,27,28,29,30,31,32]. However, other studies suggested that dying-back theory did not completely explain the ACR-induced neuropathy.…”

Stereological and allometric studies on neurons and axo-dendritic synapses in the superior cervical ganglia of rats, capybaras and horses

“…In order to produce vertical and uniform random (VUR) sections (Baddeley et al 1986;Tandrup 1993;Tandrup and Braendgaard 1994), SCGs from rats, capybaras and horses were rotated along their own long axis by using a bar rotator and their vertical axes were marked by using a Tissue Marking Dye System (Cancer Diagnostics). SCGs from capybaras and horses were embedded in a 6% agar solution and, by using a tissue slicer, cut into 1-mm-thick slabs by using a systematic uniform random (SUR) sampling scheme.…”

2,3-Dihydroxybenzoic acid attenuates kanamycin-induced volume reduction in mouse utricular type I hair cells

“…It is important to note that cell volume is often a better pathological parameter than cell number, since changes can be observed long before the cells actually die and disappear (Janson and Møller, 1993;Tandrup and Braendgaard, 1994). It is not known whether the affected HCI in the present study are dying, but as shown in a previous study there .99] · 10 À3 mm 3 in control animals, 3.14 · 10 À3 mm 3 (0.04) [2.93; 3.2] · 10 À3 mm 3 in kanamycin-treated animals and 3.50 · 10 À3 mm 3 (0.06) [3.20; 3.79] · 10 À3 mm 3 in kanamycin + DHB-treated animals.…”