Number of biopsied trophectoderm cells is likely to affect the implantation potential of blastocysts with poor trophectoderm quality

Zhang, Shuoping; Luo, Keli; Cheng, Dehua; Tan, Yue‐Qiu; Lu, Can‐Zhong; He, Hua; Gu, Yifan; Lu, Guangxiu; Gong, Fei; Lin, Ge

doi:10.1016/j.fertnstert.2016.01.011

Cited by 69 publications

(44 citation statements)

References 31 publications

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“…Biopsy of cleavage-stage embryos and trophectoderm cells has been associated with reduced implantation potential possibly caused by embryonic damage (Cohen et al, 2007;Zhang et al, 2016). All embryos in our study underwent ICSI, polar-body biopsy and assisted hatching; no additional invasive procedure potentially impairing the embryo has been applied in this study.…”

Section: Discussionmentioning

confidence: 99%

Non-invasive preimplantation genetic screening using array comparative genomic hybridization on spent culture media: a proof-of-concept pilot study

Feichtinger

Vaccari

Carli

et al. 2017

Reproductive BioMedicine Online

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The aim of this pilot study was to assess if array comparative genomic hybridization (aCGH), non-invasive preimplantation genetic screening (PGS) on blastocyst culture media is feasible. Therefore, aCGH analysis was carried out on 22 spent blastocyst culture media samples after polar body PGS because of advanced maternal age. All oocytes were fertilized by intracytoplasmic sperm injection and all embryos underwent assisted hatching. Concordance of polar body analysis and culture media genetic results was assessed. Thirteen out of 18 samples (72.2%) revealed general concordance of ploidy status (euploid or aneuploid). At least one chromosomal aberration was found concordant in 10 out of 15 embryos found to be aneuploid by both polar body and culture media analysis. Overall, 17 out of 35 (48.6%) single chromosomal aneuploidies were concordant between the culture media and polar body analysis. By analysing negative controls (oocytes with fertilization failure), notable maternal contamination was observed. Therefore, non-invasive PGS could serve as a second matrix after polar body or cleavage stage PGS; however, in euploid results, maternal contamination needs to be considered and results interpreted with caution.

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Section: Discussionmentioning

confidence: 99%

Non-invasive preimplantation genetic screening using array comparative genomic hybridization on spent culture media: a proof-of-concept pilot study

Feichtinger

Vaccari

Carli

et al. 2017

Reproductive BioMedicine Online

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“…Another study confirmed that when there was no difference on embryologists performing TE biopsy at blastocysts stages in a standardized IVF setting, there was high consistency and reproducibility of CSS with optimal genetic and clinical outcomes [22]. However, a study has shown that TE quality may affect implantation potential [23] and more importantly that a large amount of TE cells biopsied may affect pregnancy rates [24].…”

Section: Introductionmentioning

confidence: 92%

The number of biopsied trophectoderm cells may affect pregnancy outcomes

Guzmán¹,

Nuñez²,

López³

et al. 2018

J Assist Reprod Genet

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Objective To study if the number of trophectoderm (TE) biopsied cells has an impact on implantation rates. Design A retrospective cohort study in a single-center study. Setting In vitro fertilization center. Patients Patients who underwent PGT-A from January 2013 to March 2016. In total, 482 vitrified/warmed single embryo transfers were included. Interventions None. Main outcome measures Clinical pregnancies rate, implantation rate. Results Overall, clinical pregnancies per embryo transfer were higher when a regular TE were biopsied compared to larger size biopsy cells (66% (175/267) vs 53% (115/215) (p < 0.005) respectively). Pregnancy rates were also analyzed according to embryo morphology at the moment of embryo biopsy, when a good-quality embryo was transferred the clinical outcome was 75% (81/108) in group 1 and 61% (60/99) in group 2 (p < 0.05). Data was also stratified by age in patients ≤ 35 years and > 35 years. The clinical pregnancy was 67% (51/76) in women ≤ 35 years and 65% (124/191) in women > 35 years when a regular size biopsy was performed. These results significantly reduced when a larger size biopsy was performed 54% (49/91) and 53% (66/124), respectively (p < 0.05). Further investigation indicated that miscarriage rate was similar between these groups (4% (7/182) in group 1 and 5% (6/121) in group 2). Conclusions These findings underscore that when a large amount of TE cells are biopsied, it may negatively affect implantation rates, but once implanted, the embryos have the same chance to miscarry or reach term.

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“…TE biopsy is technically challenging and invasive [ 12 , 13 ].TE biopsy samples typically contain about 4 to 6 cells. Increasing the number of biopsied cells might improve accuracy, but is likely to reduce implantation rate [ 14 ].Non-invasive preimplantation genetic screening (NIPGS) offers an alternative non-invasive approach which avoids embryo biopsy [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of a novel non-invasive preimplantation genetic screening approach

et al. 2018

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ObjectiveTo assess whether embryonic DNA isolated from blastocyst culture conditioned medium (BCCM) combined with blastocoel fluid (BF) could be used for blastocyst stage non-invasive preimplantation genetic testing for chromosomal aneuploidy (non-invasive preimplantation genetic screening, NIPGS).Patients47 embryos from 35 patients undergoing IVF.InterventionsDNA analysis of combined BCCM plus BF in comparison with trophectoderm (TE) biopsy and/or whole blastocyst (WB)using next generation sequencing (NGS).ResultsEmbryonic DNA was successfully amplified in 47/47 NIPGS samples (28 frozen-thawed and 19 fresh culture samples) ranging from 6.3 to 44.0 ng/μl. For frozen-thawed embryos, the concordance rate for whole chromosome copy number per sample was equivalent between NIPGS vs. TE biopsy, NIPGS vs. WB and TE vs. WB samples taken from the same embryo was 87.5%; 96.4% and 91.7% respectively (P>0.05), and the rate of concordance per single chromosome was 99.3%, 99.7% and 99.7%, respectively (P>0.05). In fresh cases (Day 4 to Day 5/6 culture), the concordance rate for whole chromosome copy number per sample between NIPGS vs. TE samples taken from the same embryo was 100%, and the rate of concordance per single chromosome was 98.2% (P>0.05).ConclusionsA combination of BCCM and BF contains sufficient embryonic DNA for whole genome amplification and accurate aneuploidy screening. Our findings suggest that aneuploidy screening using BCCM combined with BF could potentially serve as a novel NIPGS approach for use in human IVF.

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Number of biopsied trophectoderm cells is likely to affect the implantation potential of blastocysts with poor trophectoderm quality

Abstract: The implantation potential is negatively affected by the biopsied TE cell number in blastocysts with poor TE morphological score.

Cited by 69 publications

References 31 publications

Non-invasive preimplantation genetic screening using array comparative genomic hybridization on spent culture media: a proof-of-concept pilot study

Non-invasive preimplantation genetic screening using array comparative genomic hybridization on spent culture media: a proof-of-concept pilot study

The number of biopsied trophectoderm cells may affect pregnancy outcomes

Evaluation of a novel non-invasive preimplantation genetic screening approach

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