Number of interleukin‐4‐ and interferon‐γ‐secreting human T cells reactive with tetanus toxoid and the mycobacterial antigen PPD or phytohemagglutinin: distinct response profiles depending on the type of antigen used for activation

ElGhazali, Gehad; Paulie, Staffan; Andersson, Gunnel; Hansson, Yngve; Holmquist, Göran; Sun, Jiabin; Olsson, Tomas; Ekre, H P; Troye‐Blomberg, Marita

doi:10.1002/eji.1830231103

Cited by 99 publications

(56 citation statements)

References 40 publications

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“…ElGhazali vaccination and reported both IFN-g and IL-4 induction by the ELISPOT technique [13]. They suggest that after a booster nonspecific T cells are also activated because this induction declines after 1 week.…”

Section: Discussionmentioning

confidence: 97%

Interferon‐γ Production in Antigen Specific T Cell Response: Quantitation of Specific mRNA and Secreted Protein

et al. 1997

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Halminen M, Klemetti P, Vaarala O, Hurme M, Ilonen J. Interferon-g Production in Antigen Specific T Cell Response: Quantitation of Specific mRNA and Secreted Protein. Scand J Immunol 1997;46:388-392 Interferon gamma (IFN-g) production as a measure of cellular sensitization was studied by detection of the cytokine in culture supernatant by enzyme immunoassay (EIA) and by measuring cellular mRNA using the reverse transcriptase polymerase chain reaction (RT-PCR) method. These assays were compared to the standard lymphocyte proliferation assay as a marker of T cell responsiveness to foreign antigens. When blood donors seropositive for herpes simplex virus (HSV) were compared to seronegative donors, all measurements of cellular sensitization separated the groups without overlap. There were significant correlations between the IFN-g mRNA titre and the secreted IFN-g (r ¼ 0.57, P ¼ 0.03), and the proliferative response and the secreted IFN-g (r ¼ 0.78, P ¼ 0.001), as well as between the IFN-g mRNA titre and the proliferative response (r ¼ 0.78, P < 0.001). When tetanus toxoid (TT) responses were studied in immunized subjects, a wide range of responsiveness could be seen and correlation between various measurements was poor. However, constant individual levels of the cytokine production were demonstrated. Six people who had received their last TT booster vaccination more than 5 years ago were revaccinated and repeatedly studied. An increase in the levels of produced IFN-g could be seen in all subjects and two who lacked a lymphocyte proliferation response developed it after revaccination.

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Section: Discussionmentioning

confidence: 97%

Interferon‐γ Production in Antigen Specific T Cell Response: Quantitation of Specific mRNA and Secreted Protein

et al. 1997

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“…The ELISPOT assay was performed as described previously. 18 Briefly, 100 l of monoclonal antibody (MAb) to human IFN-␥ (1-D1K) or IL-4 (82-4) (Mabtech AB) were adsorbed to nitrocellulose plates (Millititer, Millipore Corp., Bedford, MA) at a concentration of 15 g/ ml in sterile phosphate-buffered saline (PBS). The plates were incubated overnight at 4ЊC and washed with PBS.…”

Section: Methodsmentioning

confidence: 99%

“…For this purpose, peripheral blood mononuclear cells (PBMC) were evaluated for their capacity to proliferate as well as to secrete interferon-␥ (IFN-␥) and/or interleukin-4 (IL-4) in response to the EB200-derived peptides as measured by the ELISPOT assay. 18 To investigate the relationship between B and T cell responses, serum was analyzed for antibody reactivity with recombinant EB200 and EB200-derived peptides.…”

mentioning

confidence: 99%

Human immune responses to the highly repetitive Plasmodium falciparum antigen Pf332.

Kulane

Siddique²,

Sarthou³

et al. 1999

The American Journal of Tropical Medicine and Hygiene

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Abstract. The B and T cell responses to EB200, a repetitive part of the Plasmodium falciparum antigen Pf332, were examined in malaria-exposed Senegalese adults. Most donors had high levels of antibodies to recombinant EB200 and 17 overlapping peptides spanning EB200. Taking proliferation and/or cytokine (interferon-␥ and interleukin-4) production as a measure of T cell activation, eight of the EB200-derived peptides induced responses in Ͼ 40% of the donors tested. There was no general association between the different types of T cell responses measured, emphasizing the importance of including multiple parameters when analyzing T cell responses and suggesting that EB200 induces functionally distinct T cell responses. The most efficient peptide for induction of proliferative responses was one previously shown to induce T cell responses in five different H-2 congenic mouse strains primed with EB200, suggesting that this is a universal T cell epitope. The presence of multiple B and T cell epitopes in EB200, widely recognized by humans, is important since EB200 has been shown to elicit protective antibody responses in monkeys and may be considered for inclusion in malaria subunit vaccines.The Plasmodium falciparum antigen Pf332 is of potential interest for inclusion in a subunit vaccine against the malaria parasite.

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“…ELISPOT assay IFN-g-and IL-4-secreting cells were detected by ELISPOT assay as previously described [18]. Briefly, 100 ml of TCM containing 2 × 10 5 PBMC (pre-cultured in presence or absence of malaria antigens or mitogen for 3-4 h) were dispensed into triplicate wells of nitrocellulose-bottomed plates (Millipore Co., Bedford, MA) precoated with anti-IFN-g MoAb 1-DIK or anti-IL-4 MoAb 82-4 (15 mg/ml) (Mabtech AB, Stockholm, Sweden).…”

Section: Estimation Of Il-4 and Ifn-g Production At The Single Cell Lmentioning

confidence: 99%

Elevated plasma levels of IgE in Plasmodium falciparum-primed individuals reflect an increased ratio of IL-4 to interferon-gamma (IFN-γ)-producing cells

ElGhazali

Perlmann

Rutta

et al. 1997

Clinical and Experimental Immunology

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SUMMARYPeople living in Plasmodium falciparum-endemic areas frequently have elevated levels of total as well as P. falciparum-specific serum IgE. This study aimed at investigating whether the elevated serum IgE levels reflect a shift in the balance between CD4 + T helper 1 (Th1) and T helper 2 (Th2) cells in individuals naturally exposed to the P. falciparum parasite. To investigate the role of Th1 and Th2 cells in the human P. falciparum system we used the ELISPOT assay to determine the ratio of IFN-g-and IL-4-producing cells after specific antigen or mitogen activation in vitro. The donors were individuals who had acquired immunity through natural exposure to the parasite. In response to the specific malaria antigens, very few IL-4-producing cells were seen. However, in the response of individual donors to the polyclonal T cell activator, leucoagglutinin (La), the anti-malarial IgE levels in plasma were correlated with an increased ratio of IL-4/IFN-g producing cells. Thus, donors with ratios of IL-4/IFN-g > 1 exhibited mean plasma anti-malarial IgE levels significantly greater than those with ratios < 1. In individuals not living in P. falciparum-endemic areas the ratio of IL-4/IFN-g was always < 1. Taken together, our data suggest a shift in the balance between Th1 and Th2 cells in naturally P. falciparumprimed individuals, associated with elevated anti-P. falciparum plasma IgE levels. The role and biological significance of IgE (Th2-type immune response) for protection against P. falciparum and/or pathogenesis of malaria require further study.

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Number of interleukin‐4‐ and interferon‐γ‐secreting human T cells reactive with tetanus toxoid and the mycobacterial antigen PPD or phytohemagglutinin: distinct response profiles depending on the type of antigen used for activation

Cited by 99 publications

References 40 publications

Interferon‐γ Production in Antigen Specific T Cell Response: Quantitation of Specific mRNA and Secreted Protein

Interferon‐γ Production in Antigen Specific T Cell Response: Quantitation of Specific mRNA and Secreted Protein

Human immune responses to the highly repetitive Plasmodium falciparum antigen Pf332.

Elevated plasma levels of IgE in Plasmodium falciparum-primed individuals reflect an increased ratio of IL-4 to interferon-gamma (IFN-γ)-producing cells

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