Numbers of natural killers lymphocytes do not determine their cytotoxicity

Dons’koi, Boris V.

doi:10.30970/sbi.1302.599

Cited by 2 publications

(1 citation statement)

References 30 publications

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“…That is, the accented parameter creates difficulties for adapting the system to new challenges. We separately investigated different NK cytotoxicity and levels of NK cells and found that the zones where these factors are unfavorable (too high and too low) are precisely the zones where these parameters are highly reliably correlated with each other [17] and in the reproductively favorable zone there is no correlation between them [18]. That is, in favorable zones, the number of NK cells does not determine the function of NK cells.…”

Section: Introductionmentioning

confidence: 98%

Theory of immune accentuations: formation of accentuated immunophenotype

Dons’koi

2023

Lik. sprava

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In numerous previous studies, we have formulated the idea of immune accentuation, demonstrated associations between immune accentuations and the unfavorable course of physiological processes, and shown that the association of several accentuations has a reliable negative effect on the further course of the reproductive process. In the article, we demonstrated the correlative and associative nature of certain immune accentuations, which explains the causes of the formation of an unfavorable immuno-accentuated phenotype. Thus, we found highly reliable correlative and associative relationships between the parameters of natural resistance (NK quantity, NK cytotoxicity, expression of CD158a and CD8 on NK cells), all these parameters were mutually correlated with each other and their accentuations were associated with accentuations of partner parameters. Another immuno-accented cluster had pro-inflammatory features, so the expression of HLA-DR on NK and T-cytotoxic cells, as well as NKT-like populations (CD3+CD56+ and CD3+CD158a+) also had highly reliable correlations and associations. This pro-inflammatory phenotype was negatively correlated and inversely associated with T-helper levels. Presumably, this is a consequence of uncontrolled chronic activation with the appearance of alternative ways of differentiation of T cells that bypass regulatory control by T-helpers. We showed a fundamental difference in the association and correlative relations between immune parameters depending on their level. Thus, HLA-DR levels on NK cells within (NK < 24 %) had a negative correlation with % NK cells, while, within (NK > 24 %) they begin to correlate positively and reliably with the number of NK. So, accentuation is the place in the distribution of the parameter, where the parameter begins to put pressure on other parameters and determine their level of direction. These are the same zones where the parameter becomes unfavorable, because it loses the possibility of regulation. Accentuation of the immune parameter is associated with specific accentuations of other parameters. Whether it causes changes in other links or whether it and other changes are the result of extra-immune or environmental factors are unknown. But it is clear that this leads to the formation of an immuno-accented phenotype. Accentuation creates prerequisites for the formation of other immune accentuations due to the imbalance of systemic immune regulation.

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Section: Introductionmentioning

confidence: 98%

Theory of immune accentuations: formation of accentuated immunophenotype

Dons’koi

2023

Lik. sprava

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Immune factors and health of Antarctic explorers

Kozeretska

Deineko

Anoshko

et al. 2021

UAJ

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The immune system plays a major role in human homeostasis, yet a body’s unique individuality complicates the diagnostic forecasting of unfavourable physiological states and diseases. Studying the immunophenotypic features of winterers of the Ukrainian Antarctic Expeditions before, during, and after their assignments might shed some light on the possible place of immune accentuations in the development of certain physiological states. To determine the natural-killer (NK) cytotoxicity and the immunophenotype in 52 applicants who wanted to take part in an expedition and nine participants who had come back, we used flow cytofluorometry. Blood serum samples taken before, during, and after the expeditions were also tested for hormones, anti-infective, anti-parasitic, and autoimmune antibodies. The high absolute and relative numbers of NK lymphocytes, high NK cytotoxicity, and high expression of HLA-DR on the CD3+CD8+ lymphocytes were correlated with a person’s unfavorable health status during the expedition. In Antarctica, cortisol levels sharply increased, yet they normalized upon return. In most winterers, there were no significant health complications during the expeditions. Neither reactivated nor primary viral infections were registered, as well as clinical autoimmune ones. Upon return, the winterers had significantly lower leukocytes and lymphocytes and increased expression of activation markers (HLA-DR) on the T-cells. The found risk factors can characterize the polar researchers’ immunophenotypes yet require validation on larger samples. The expedition environment causes increased stress, entailing, however, neither clinical manifestations nor elements of immunosuppression. The polar researchers bear the consequences of the prolonged stress that inhibit leucopoiesis as late as six months after their return, which should be considered while reviewing applications for the next season.

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Numbers of natural killers lymphocytes do not determine their cytotoxicity

Cited by 2 publications

References 30 publications

Theory of immune accentuations: formation of accentuated immunophenotype

Theory of immune accentuations: formation of accentuated immunophenotype

Immune factors and health of Antarctic explorers

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