Numerical analysis of an enhanced cooling rate cryopreservation process in a biological tissue

Sukumar, S.; Kar, Satya Prakash

doi:10.1016/j.jtherbio.2019.03.001

Cited by 8 publications

(8 citation statements)

References 26 publications

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“…On sustaining freezing for a little beyond 7 s, the whole transect attains our desired temperature. It must be realized that merely freezing the sample would cause little benefit in preservation unless frozen up to 77.15 K. 3,9 The constant temperature cooling condition of 77.15 K shows the most drastic thermal behavior within the tissue sample. The complete transect is seen to freeze between 1 s and 3 s with a sight of almost a linear curve of temperature between the axis and cold wall near the required LN 2 freezing temperature.…”

Section: Acquired Sample (In Vivo)mentioning

confidence: 99%

“…The present scope of work encompasses the macroscale‐study of conventional cryopreservation on an acquired tissue sample during needle‐based biopsy. Recently, Sukumar and Kar 9 applied the finite volume method to study the conventional LN 2 probe‐based cryopreservation process with enhanced cooling rate in soft biological tissue. Effects of single‐side and both‐side freezing were observed with a note on the consequence of blood perfusion rate in freezing.…”

Section: Introductionmentioning

confidence: 99%

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Thermal modeling of a dual‐purposed snap biopsy acquisition procedure in a suspected cancerous lesion

et al. 2023

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The needle‐based biopsy procedure is common in cancer detection and patient‐specific targeted therapy, wherein a tissue sample from the potential diseased site is acquired and frozen instantly with the help of a coolant medium. While liquid nitrogen (LN2) is the most widely used coolant for preserving the acquired sample and performing biopsy tests on the same at a later time, cold ischemia leads to inevitable cell degradation beyond a threshold time. In an effort to circumvent this challenge, here we aim to put forward the concept of an integrated biopsy sample acquisition and cryotherapy procedure, by incorporating an exclusively designed cooling circuit in a conventional biopsy‐needle for freezing the sample in vivo as soon as it is acquired, while causing cryoablation in the surrounding tissues simultaneously. An enthalpy‐based approach is employed to develop a bioheat transfer model for the cryotherapy design, with illustrative simulation data presented for breast cancer. Our model is demonstrated by considering a constant LN2 cooling temperature of 77.15 K, and cooling powers ranging from 2 to 10 W. The model results elucidate procedure‐specific insights such as the thermal penetration depth and the cooling time on being subjected to the cryoablation. The cooling rates thus obtained are further assessed from the simultaneous considerations of cryopreservation and cryosurgery, deriving critical insights on tissue survival and damage for acting as a precursor to patient‐specific treatment planning.

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Section: Acquired Sample (In Vivo)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Thermal modeling of a dual‐purposed snap biopsy acquisition procedure in a suspected cancerous lesion

et al. 2023

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“…The rate of cooling imposed during the cryopreservation process greatly determines the cell survival outcome [60][61][62]. The effect can be explained by an underlying principle described as the inverted "U" of cryobiology (Figure 7).…”

Section: Applications Of Biophysics Understanding 91 Selection Of Coo...mentioning

confidence: 99%

Biophysics of Cryopreservation

SHARMA

2022

International Journal of Thermodynamics

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A significant credit towards today's scientific and medical advancements goes to the technique of cryopreservation. Cryopreservation refers to the maintenance of cellular life at subzero temperatures for a definite period of time in a state of suspended cellular metabolism. The technique has become an indispensable step in most scientific research and medical applications like assisted reproduction, transplantations, and cell-based therapies where-in it allows the long-term preservation of biological specimens like gametes, embryos, viruses, cells and tissues. Although already an extensively used technique, a significant proportion of the cryopreserved samples still incur notable damage. Ultimately this leads to a decreased post-thaw viability and proliferation. Moreover, it is also possible that events during the freezing process, provoke more serious disturbances in the preserved material with regard to its identity and functionality. Hence, with the need to use the technique more judiciously, additional studies are needed for optimizing the current cryopreservation methods in use. For this, a thorough understanding of the normal physiological changes that the cryopreserved sample undergoes and the physics of cryopreservation seems plausible. The review thus aims to unravel the current knowledge on the complex physico-chemical processes and reactions that occur during the standard cryopreservation techniques.

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“…Majumdar and Kumar 10 found the analytical solution of the 1D transient Pennes bio-heat transfer equation using the coordinate transformation technique during cryosurgery. It is to be noted here that a single cryoprobe was used in these studies [6][7][8][9][10] to destroy the cancerous cells.…”

Section: Introductionmentioning

confidence: 99%

“…They simulated the problem using commercial software FLUENT 5.0 to determine the temperature distribution inside the biological tissue during cryosurgery. Sukumar and Kar 8 numerically investigated the effect of cryopreservation in two‐dimensional (2D) biological tissue using the finite volume method. They found greater cooling rates and shorter freezing times in the case of both‐side freezing compared to single‐side freezing.…”

Section: Introductionmentioning

confidence: 99%

Numerical analysis of phase‐change heat transfer in axisymmetric biological tissue during nano‐cryosurgery

Kumar

2023

Heat Trans

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The current work is concerned with the numerical investigation of phase-change heat transfer in a cylindrical-shaped biological tissue during nanocryosurgery. So, the two-dimensional axisymmetric biological tissue with a single cryoprobe is considered to understand the freezing process inside it during nano-cryosurgery. This problem has been numerically simulated using the commercial software ANSYS Fluent 2020 R2. The results obtained are verified with the data available in the literature, and good agreement was found between them. After that, the gridindependent study is carried out to select the optimum element size. Then, the effect of various parameters, such as the radius and insertion depth of the cryoprobe and internal heat source, on the temperature distribution inside the biological tissue is studied. The effect of different nanoparticles, such as Au, Fe 3 O 4, and MgO, on the temperature field are investigated, and it was found that the MgO nanoparticles reach the threshold temperature limit early and reduce cryoinjury to the surrounding healthy tissue. This study may help understand the freezing process inside the biological tissue during nano-cryosurgery.

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Numerical analysis of an enhanced cooling rate cryopreservation process in a biological tissue

Cited by 8 publications

References 26 publications

Thermal modeling of a dual‐purposed snap biopsy acquisition procedure in a suspected cancerous lesion

Thermal modeling of a dual‐purposed snap biopsy acquisition procedure in a suspected cancerous lesion

Biophysics of Cryopreservation

Numerical analysis of phase‐change heat transfer in axisymmetric biological tissue during nano‐cryosurgery

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