Numerical analysis of electrophoretic protein patterns of Bacteroides ureolyticus clinical isolates

Abstract

A total of 37 Bacteroides ureolyticus clinical isolates (mainly from the United Kingdom) comprising 18 nongonococcal urethritis-associated strains and 19 other strains, including the type strain (NCTC 10941T) and strains from abscesses, ulcers, and various other soft tissue lesions, and five reference strains of other Bacteroides, Fusobacterium, Megamonas, and Sebaldella species were characterized by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis of cellular proteins. The protein patterns, which contained 45 to 50 discrete bands, were highly reproducible and were used as the basis for a numerical analysis. Four clusters comprising 31 strains of B. ureolyticus were obtained at the 70% similarity level, and one of these was further divided to give two subclusters at the 82% similarity level. The analysis showed that the clusters obtained corresponded with three previously identified polyacrylamide gel electrophoresis types (I, II, and III) within the species, although two slightly different patterns within type I were detected. Evidence is presented for a new protein pattern (type IV) which was characteristic of NCTC 10941T and seven isolates. There was no clear-cut correlation between the pattern type and the clinical source of the isolates, except for the type Mb and type IV strains, which were nongonococcal urethritis-associated and wound isolates, respectively.

Reclassification of Bacteroides ureolyticus as Campylobacter ureolyticus comb. nov., and emended description of the genus Campylobacter

“…Supplementary Figure S1 shows the result of the numerical comparison of the protein profiles of the 26 strains. As discussed by Taylor et al (1986Taylor et al ( , 1987 the whole-cell protein profiles of B. ureolyticus strains differ mainly in the position of major protein bands in the molecular mass region of 36-52 kDa (Taylor et al, 1987). The same observations have been reported for a range of species of the genus Campylobacter (e.g.…”

Numerical analysis of electrophoretic protein patterns of Providencia rettgeri strains from human faeces, urine and other specimens

“…Typing by PAGE overcomes such problems especially as the same approach can be used for other organisms (which for serotyping would each require a large range of sera). Other organisms to which PAGE typing has been applied include Acinetobacter (Alexander et al 1984), Bacteroides (Taylor et al 1987), Campylobacter (Costas et al 1987b), Clostridium (Tabaqchali et al 1986) and Streptobacillus .…”

Numerical analysis of electrophoretic protein patterns of Providencia rustigianii strains from human diarrhoea and other sources

“…The use of computerized PAGE of proteins in the identification of clinically significant strains at the species level has, as yet, found little application. Protein patterns offer considerable potential for typing strains of clinical interest, however, especially for species for which no other typing methods are available (Alexander et al 1984;Tabaqchali et al 1986;Costas & Owen 1987;Costas et al 1987;Taylor et al 1987). The aim of the present study was to compare the PAGE protein patterns of Prou.…”