Numerical Analysis of Enzyme Polymorphism: A New Approach to the Epidemiology and Taxonomy of Trypanosomes of the Subgenus Trypanozoon

Gibson, Wendy; Marshall, Tom; Godfrey, D.G.

doi:10.1016/s0065-308x(08)60400-5

Cited by 229 publications

(160 citation statements)

References 67 publications

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“…Conventional starch gel stains usually involve volumes of 25 to 50 ml, which is about one order more than is required for staining other gels of the same size. Starch gel is more friable than polyacrylamide gels and is not easy to handle (Gibson, et al, 1980). Flint, et al, (2008), have studied comparison between starch and polyacrylamide gels for the analysis of Listeria monocytogenes using multilocus enzyme electrophoresis.…”

Section: It Is Chemically Inertmentioning

confidence: 99%

Enzyme Electrophoresis in Shrimps by Polyacrylamide Gel for Phylogeny: A Practical Guide

M¹,

Shakunthala²,

Ramesh³

2010

MAS

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Polyacrylamide electrophoresis gel for studying multilocus enzyme uses the relative electrophoretic mobilities of intracellular enzymes to characterize and differentiate organisms by generating an electromorph type. There are many articles for enzyme electrophoresis by using starch gel but there is lacuna by using polyacrylamide gel especially in shrimps. This article presents the chemical conditions for some enzyme that may be useful, a guide to the successful practice of the electrophoretic technique, by using polyacrylamide gel in shrimps.

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Section: It Is Chemically Inertmentioning

confidence: 99%

Enzyme Electrophoresis in Shrimps by Polyacrylamide Gel for Phylogeny: A Practical Guide

M¹,

Shakunthala²,

Ramesh³

2010

MAS

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“…The existence of genetic exchange between T. brucei trypanosomes could account for the evolution of extensive molecular variation and polymorphism observed (Gibson et al 1980). Direct observations of meiosis and mitosis are lacking as trypanosome chromosomes do not condense throughout the cell division cycle and thus cannot be visua lized by classical cytological methods.…”

Section: Reproduction and Sexuality In African Trypanosomesmentioning

confidence: 99%

“…Direct observations of meiosis and mitosis are lacking as trypanosome chromosomes do not condense throughout the cell division cycle and thus cannot be visua lized by classical cytological methods. However, indirect evidence of genetic exchange has been obtained from the analysis of enzyme electrophoretic variation between natural parasite populations from field isolations (Gibson et al 1980, Tait 1980. Tait (1983) compared the observed and expected frequencies of iso-enzyme patterns of T. brucei populations and he found suggestive evidence for the exis tence of a gene exchange system.…”

Section: Reproduction and Sexuality In African Trypanosomesmentioning

confidence: 99%

“…Three independent series of experiments have revealed that T. brucei organisms are diploid for most genes. This conclusion is based on the analysis of enzyme electropho retic banding patterns (Gibson et al 1980, Tait 1980, the kinetic complexity of the genome and the measured DNA content (Borst et al 1982) as well as on the data by Gibson et al (1985) on the analysis of restriction fragment length polymorphisms (RFLP's) of single copy genes.…”

Section: Reproduction and Sexuality In African Trypanosomesmentioning

confidence: 99%

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Sexual stages in trypanosomes and implications

Jenni

1990

Ann. Parasitol. Hum. Comp.

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The basis for concluding that a system of genetic exchange exists in African trypanosomes of the Trypanosoma brucei group is out lined by the accumulated data from variation in natural parasite populations and from laboratory crossing experiments. The results indicate that these parasites can complete their life cycle either with or without genetic exchange. This sexual event involves meiosis and syngamy, but the order of these processes and the sexual stages involved are not yet known. Reproduction and sexuality in African trypanosomes

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“…For MLEE, several protocols were described, using either starch or acetate gel, studying a range of metabolic enzymes called isoenzymes (e.g. Gibson et al, 1980, Truc et al, 1991. A recent short review has been published about molecular biology and identification of trypanosomes (Truc and Cuny, 2000).…”

mentioning

confidence: 99%

About Trypanosoma brucei gambiense, the causative agent of the chronic form of Human African Trypanosomiasis: some findings and proposals

Truc¹

2003

Afr. J. Biotechnol.

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Since its discovery at the beginning of 20th century, Human African Trypanosomiasis or sleeping sickness has killed several hundred thousands of individuals in Africa, mainly in Central part of the continent. The actual situation of the disease in several countries is dramatic, such as in Sudan, Democratic Republic of Congo or Angola. However, diagnosis is still based on the detection of parasites in blood or lymphatic juice by microscopy, like in 1930's. Furthermore, since the discovery of melarsoprol in 1949, no novel drugs have been synthesized for treatment. This must consider it within the whole context of "most-neglected diseases" but some findings obtained through biotechnology are discussed in terms of epidemiology and control applications.

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Numerical Analysis of Enzyme Polymorphism: A New Approach to the Epidemiology and Taxonomy of Trypanosomes of the Subgenus Trypanozoon

Cited by 229 publications

References 67 publications

Enzyme Electrophoresis in Shrimps by Polyacrylamide Gel for Phylogeny: A Practical Guide

Enzyme Electrophoresis in Shrimps by Polyacrylamide Gel for Phylogeny: A Practical Guide

Sexual stages in trypanosomes and implications

About Trypanosoma brucei gambiense, the causative agent of the chronic form of Human African Trypanosomiasis: some findings and proposals

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