This paper presents experimental determination of the heat transfer coefficient and the friction factor in an internally rifled tube. The experiment was carried out on a laboratory stand constructed in the Department of Energy of the Cracow University of Technology. The tested tube is used in a Polish power plant in a supercritical circulating fluidized bed (CFB) boiler with the power capacity of 460 MW. Local heat transfer coefficients were determined for Reynolds numbers included in the range from ~6000 to ~50,000, and for three levels of the heating element power. Using the obtained experimental data, a relation was developed that makes it possible to determine the dimensionless Chilton–Colburn factor. The friction factor was also determined as a function of the Reynolds number ranging from 20,000 to 90,000, and a new correlation was developed that represents the friction factor in internally ribbed tubes. The local heat transfer coefficient and the friction factor obtained during the testing were compared with the CFD modelling results. The modelling was performed using the Ansys Workbench application. The k-ω, the k-ε and the transition SST (Share Stress Transport) turbulence models were applied.