Due to the increased demand for global transportation needs, a long-range diesel engine is considered an important prime mover to fulfill the transportation demand. The major problem addressed by the diesel engines is it liberates harmful emissions and it also increases global warming. CO2 is considered an important greenhouse gas and it has to be controlled by diesel engines. In this research, extensive experimental work is done to identify a suitable solution to control CO2 emissions. For five different mole concentrations (0.1 to 0.5), three types of amino solutions (L-alanine, L-aspartic acid, and L-arginine) were prepared. By passing diesel exhaust through each solution, CO2 absorption is investigated. For CO2 absorption, an appropriate CO2 control system is built and tested. The tests were carried out in a diesel engine which is a naturally aspirated single-cylinder engine with a water-cooled system. It was possible to obtain an effective CO2 reduction of up to 90%, as well as a simultaneous reduction in HC and CO.