Numerical areal density of CD34 and CD117 immunoreactive hematopoietic cells in human fetal and embryonic liver

Mašić, Jelena Vladičić; Nikolić, Ivan; Todorovic, Vesna; Jović, Marko; Petrovič, Vladimír; Mašić, Srđan; Đukić, Nikolina; Zečević, Snežana

doi:10.7251/bii1902111v

Cited by 2 publications

(1 citation statement)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Antibody unmasking was performed for 20 min in citrate buffer at pH 6.0. Thereafter, tissue sections were incubated with a 3% hydrogen peroxide solution for 10 minutes at room temperature to block endogenous peroxidase activity [16]. Phosphate buffer (Dako, EnVsion FLEX WASH BUFER) was used to wash the tissue section three times for two minutes.…”

Section: Methodsmentioning

confidence: 99%

Analysis of CD31 expression and vascular parameters in human placentas from pregnant women with intrauterine growth restriction

Ljubojević¹,

Jovičić²,

Draganović³

et al. 2022

Biomedicinska istraž.

View full text Add to dashboard Cite

Introduction. Placental dysfunction is underlying cause in most of the intrauterine growth restriction and the pregnancy complications where the fetus does not achieve its genetically determined potential for growth. The critical process for the development of the placenta is angiogenesis. CD31 is an important endothelial adhesion protein that enables angiogenesis. The study aimed to analyze the CD31 expression and vascular parameters in normal placentas and IUGR placentas. Methods. Thirty placental samples, fifteen IUGR placentas, and fifteen term normal placental samples were analyzed. The hematoxylin-eosin method and immunohistochemical method with anti CD31 antibody were used for the staining of the tissue sections. The analyzed vascular parameters were: capillary number density (CND), capillary area density (CAD), and capillary surface density (CSD). Results. Between normal placentas and IUGR placentas there was no determined difference in CD31 expression. Positive intensive staining of CD31 was found in the endothelium of all blood vessels and no staining was observed in cytotrophoblast and syncytiotrophoblast cells. In IUGR placentas, CND of 2.55 capillary/1000 µm2 villous area was significantly decreased compared to normal placentas of 3.49 capillary/1000 µm2 villous area. CAD in IUGR placentas of 30.49 % was significantly decreased compared to normal placentas of 52.80 % villous area. CSD in IUGR placentas (92.81 µm/1000µm2) was significantly reduced compared to CSD in normal placentas (145.51 µm/1000µm2). Conclusion. The localization and intensity of CD31 expression were not different between the IUGR and normal placentas. Histological vascular parameters of placental villi are decreased in the IUGR placenta. In case of intrauterine growth restriction, there is a reduced vascularization of the terminal villi of the placenta.

show abstract

Section: Methodsmentioning

confidence: 99%

Analysis of CD31 expression and vascular parameters in human placentas from pregnant women with intrauterine growth restriction

Ljubojević¹,

Jovičić²,

Draganović³

et al. 2022

Biomedicinska istraž.

View full text Add to dashboard Cite

show abstract

Effect of Lactobacillus fermentum ZS40 on the NF-κB signaling pathway in an azomethane-dextran sulfate sodium-induced colon cancer mouse model

Liu

Wang²,

Yi³

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

The occurrence of intestinal diseases such as colon cancer is closely related to the intestinal flora. Lactobacillus fermentum is a gut probiotic that plays an important role in chronic intestinal inflammation and colon cancer. In the current study, we investigated the effect of Lactobacillus fermentum ZS40 on NF-κB signaling pathway of azomethane-dextran sulfate sodium (AOM-DSS) -induced colon cancer in mice. Animals were divided into control group (NC), AOM-DSS-induced model group (CRC), AOM-DSS plus high-dose Lactobacillus fermentum ZS40 (ZS40-H), AOM-DSS plus low-dose Lactobacillus fermentum ZS40 (ZS40-L), AOM-DSS plus Lactobacillus bulgaricus (BLA), and AOM-DSS plus sulfasalazine (SD)-treated group. Observation of animal physiological activity (body weight and defecation), biochemical measurements, histopathological examination of colon tissue, qPCR to evaluate the expression of inflammation-related genes, immunohistochemical analysis of CD34 and CD117, and Western blot analysis of NF-κB signaling pathway were performed. Compared with the CRC group, the ZS40-H, ZS40-L, BLA, and SD groups had decreased levels of colon cancer marker proteins CD34 and CD117, and the number of abnormal colonic lesions observed by colon histology decreased, while the ZS40-H group showed excellent results. In addition, all probiotic interventions showed weight loss effects. The expression of inflammatory stimulators TNF-α and IL-1β in the probiotic treatment group decreased; the expression of key proteins IκBα and p65 in the NF-κB signaling pathway also decreased, resulting in a decrease in the expression of the target protein Cox-2. Therefore, administration of Lactobacillus fermentum ZS40 as a probiotic can alleviate intestinal inflammation and prevent colon cancer in mice.

show abstract

Numerical areal density of CD34 and CD117 immunoreactive hematopoietic cells in human fetal and embryonic liver

Cited by 2 publications

References 18 publications

Analysis of CD31 expression and vascular parameters in human placentas from pregnant women with intrauterine growth restriction

Analysis of CD31 expression and vascular parameters in human placentas from pregnant women with intrauterine growth restriction

Effect of Lactobacillus fermentum ZS40 on the NF-κB signaling pathway in an azomethane-dextran sulfate sodium-induced colon cancer mouse model

Contact Info

Product

Resources

About