Numerical Classification of 280 Strains of Slowly Growing Mycobacteria

Tsukamura, M

doi:10.1111/j.1348-0421.1983.tb00591.x

Cited by 39 publications

(35 citation statements)

References 34 publications

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“…africanum, as evidenced by almost identical genomic DNAs. My data also support the reclassification of these species as a single species, as suggested by numerical taxonomy (25,26).…”

Section: Discussionmentioning

confidence: 49%

Deoxyribonucleic Acid Relatedness Among Selected Strains of Mycobacterium tuberculosis, Mycobacterium bovis, Mycobacterium bovis BCG, Mycobacterium microti, and Mycobacterium africanum

Imaeda

1985

International Journal of Systematic Bacteriology

108

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Deoxyribonucleic acids (DNAs) of selected strains of the Mycobacterium tuberculosis complex, including strains of M . tuberculosis, Mycobacterium bovis, M. bovis BCG, Mycobacterium microti, and Mycobacterium africanum, were isolated and analyzed spectrophotometrically. The genome molecular weight and the guanine-plus-cytosine content of the DNA samples were approximately 1.9 X lo9 and 62.9 mol% , respectively. DNA relatedness among the strains was studied by both spectrophometric DNA hybridization and restriction endonuclease cleavage methods. All strains of the four species showed more than 90% DNA relatedness, except for slightly lower levels (85 to 89%) of relatedness between M . microti and some strains of M. bovis, M . bovts BCG, or M . africanum. The distribution patterns of the DNA fragments produced with restriction enzymes, such as EcoRI, EcoRV, BglII, BcZI, and KpnI, were indistinguishable upon agarose gel electrophoresis. In contrast, the levels of DNA relatedness between M . tuberculosis and other slowly growing mycobacteria, including Mycobacterium nonchromogenicum, Mycobacterium intracellulare, Mycobacterium kansasii, Mycobacterium avium, Mycobacterium scrofulaceum, Mycobacterium haemophilum, Mycobacterium marinum, and Mycobacterium ashticum, were significantly heterogeneous, ranging from 9 to 53%. My data indicate the extremely close evolutionary relationship among four species of the M. tuberculosis complex.Mycohacterium tuberculosis, Mycobacterium bovis, M . bovis BCG, Mycobacterium microti, and Mycohacteriirm africanum are grouped as the M. tirherculosis complex based upon their phenetic similarities (25, 26). Distinctions among these species are defined on the basis of biochemical (9, 21, 25, 26), bacteriological (7,13,21,26), serological (8, 15, 19, 22) and chemical (23, 27) properties, their levels of pathogenicity for experimental animals (10, 13, 20, 21), and their levels of susceptibility to some mycobacteriophages (6, 28). A thorough review of these taxonomic findings has been published by Wayne (26).However, any of the extensive phenotypic analyses available measure less than 20% of the total genomic capability of bacteria (4). Consequently, phenotypic differences should not be emphasized as absolute criteria for definition of species. On the other hand, examination of essential properties of chromosomes, such as genome molecular weight, guanine-plus-cytosine content, and deox yri bonucleic acid (DNA) relatedness, provides a more practical and reliable method to determine the genetic relationships among phenetically similar species. In fact, Bradley (3) and Baess (2) reported 100% DNA homology between M . tirbercirlosis and M . bovis, including M . bovis BCG strains, showing that these organisms belong to the same species.In this report I provide additional information concerning the genome molecular weights, base ratios, and extents of DNA relatedness among selected strains of the species of the M . tubercrrlosis complex. A total of 13 M . hovis BCG strains commonly used for vaccinatio...

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“…africanum, as evidenced by almost identical genomic DNAs. My data also support the reclassification of these species as a single species, as suggested by numerical taxonomy (25,26).…”

Section: Discussionmentioning

confidence: 49%

Deoxyribonucleic Acid Relatedness Among Selected Strains of Mycobacterium tuberculosis, Mycobacterium bovis, Mycobacterium bovis BCG, Mycobacterium microti, and Mycobacterium africanum

Imaeda

1985

International Journal of Systematic Bacteriology

108

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“…In laboratory media, M. avium complex organisms have a pH optimum for growth of 5 to 5.5 (170,400). M. avium strains grow at 45ЊC, unlike M. intracellulare strains, which can grow to only 42ЊC (258,430,499). From these characteristics, coupled with the relative chlorine resistance of mycobacteria (70,195), it is not surprising that M. avium complex organisms are found in municipal drinking-water systems and that M. avium is concentrated by hospital hot-water distribution systems (131,516,517).…”

Section: Physiologic Ecology Of the M Avium Complexmentioning

confidence: 99%

“…The major distinguishing characteristic of M. xenopi is its ability to grow at 45ЊC but not at 28ЊC (258,338,499). In addition, M. xenopi colonies are unique, forming irregular, stick-like projections best observed by phase-contrast microscopy (338), and isolates are arylsulfatase positive in 3 days (258).…”

Section: Characteristics Of M Xenopimentioning

confidence: 99%

“…Unlike other scotochromogens, M. szulgai hydrolyzes Tween 80 (73,258,327). M. szulgai isolates have 68ЊC-resistant catalase activity and nitrate reductase and urease activities but lack acid phosphatase activity (177,499). M. szulgai fails to grow in 5% NaCl.…”

Section: Characteristics Of M Szulgaimentioning

confidence: 99%

“…M. marinum growth does not occur above 32ЊC upon initial isolation, which distinguishes that species from M. kansasii (258). However, M. marinum strains maintained on laboratory media will grow at 37ЊC, although less luxuriantly than at 28ЊC (80,499). M. marinum lacks catalase and nitrate reductase activities, and some but not all isolates hydrolyze Tween 80 (258).…”

Section: Characteristics Of M Marinummentioning

confidence: 99%

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