Numerical Classification of the Tribe Klebsielleae

Bascomb, Shoshana; Lapage, S. P.; Willcox, W. R.; Curtis, Mavis

doi:10.1099/00221287-66-3-279

Cited by 118 publications

(62 citation statements)

References 19 publications

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“…The differentiation of Serratia by esterase profiles is consistent with the results obtained by numerical taxonomy (Bascomb et al, 1971;Grimont et al, 1977 b), DNA hybridization (Steigerwalt et al, 1976) and electrophoresis of proteases (Grimont et al, 1977~). On the basis of the esterase profiles in the present study, S. marcescens, S. liquefaciens and S. plymuthica appear to be more closely related to one another than to S. marinorubra.…”

Section: Discussionsupporting

confidence: 91%

Characterization of Serratia marcescens, S. liquefaciens, S. plymuthica and S. marinorubra by the Electrophoretic Patterns of their Esterases

Goullet

1978

Journal of General Microbiology

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Esterases of 62 Serratia marcescens, S. liquefaciens, S. plymuthica and S. marinorubra strains were analysed by horizontal slab electrophoresis in polyacrylamide-agarose gel. Five principal bands hydrolysed P-naphthyl acetate but differed in their range of activity towards other substrates and in their sensitivity to di-isofluoropropyl phosphate. Additional bands were detected in some strains of S. marcescens and S. marinorubra. The comparative distribution of bands showed that the four Serratia species were characterized by distinct electrophoretic patterns of their esterases. Serratia marcescens, S. Ziquefaciens and S. plymuthica appeared to be more closely related to one another than to S. marinorubra. In the case of S.plymuthica and S. marinorubra, congruence was found between biovars and esterase patterns. The numerous electrophoretypes observed within Serratia species might provide useful epidemiological markers. I N T R O D U C T I O NPrevious investigations have revealed that esterases of several taxa in the family Enterobacteriaceae (Goullet, 1973(Goullet, , 1975(Goullet, , 1977Goullet & Richard, 1977) give distinct and reproducible species-specific zymogram banding patterns when assayed after electrophoresis in polyacrylamide-agarose gel. In the present study, the esterases produced by the four Serratia species recognized by Grimont et al. (1977a, b) were examined. METHODSThe names and sources of the test strains are given in Table 1. Growth conditions, preparation of extracts, inhibition by di-isofluoropropyl phosphate (DFP), protein estimation, acrylamide-agarose gel electrophoresis, esterase staining and estimation of electrophoretic mobility (MF value) have all been described previously (Goullet, 1977). RESULTSThe esterase profiles resolved from multiple runs of the same and different soluble extracts from each strain were reproducible. Electrophoretic relationships were established between the strains by numerous replicate assays comparing esterase bands in adjacent positions on the same gel. Esterase bands were found in all of the test strains.Five principle esterase bands varying in sharpness and colour intensity, designated as E,, E,, ES, E, and E, in order of decreasing electrophoretic mobility, were defined ( Fig. 1 a),The El band hydrolysed a-naphthyl acetate and to a lesser degree a-naphthyl butyrate, indoxyl acetate and P-naphthyl acetate; the Ez band hydrolysed a-and P-naphthyl acetates;

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Section: Discussionsupporting

confidence: 91%

Characterization of Serratia marcescens, S. liquefaciens, S. plymuthica and S. marinorubra by the Electrophoretic Patterns of their Esterases

Goullet

1978

Journal of General Microbiology

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“…Although strains of both taxa were included in the same cluster by numerical taxonomy (Bascomb et al, 1971) it was felt inappropriate to include 'K. aerogenes' and K. oxytoca in the revised matrix as a single taxon, or even allow identification to a composite group between them, as these two taxa are so unrelated that they should, despite their close phenotypic similarity, probably be placed in separate genera (Jain et al, 1974).…”

Section: B H O L M E S C a Dawson And C A Pinningmentioning

confidence: 99%

A Revised Probability Matrix for the Identification of Gram-negative, Aerobic, Rod-shaped, Fermentative Bacteria

Holmes¹,

Dawson²,

Pinning³

1986

Microbiology

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The results of the identification of 933 strains of Gram-negative, aerobic, rod-shaped, fermentative bacteria ( Enterobacteriaceae, Pasteurellaceae, Vibrionaceae) by a probabilistic method, in a computer, are given. The identification rate on the matrix was 89.2%. Many of the strains were atypical and had caused difficulty in identification in medical diagnostic laboratories. The results are given for each taxon by genus and species. I N T R O D U C T I O NA computer-assisted conditional probability method for the identification of enterobacteria was first described by Dybowski & Franklin (1968) and Lapage et al. (1970) used a similar scheme to successfully identify up to 80% of 279 freshly isolated strains. Later, Bascomb et al. (1973) published a matrix for the identification of Gram-negative rods of clinical importance and discussed its use in the identification of 1079 reference strains; the general aspects of probabilistic identification and the mathematical model used were described by Lapage et al. (1973) and Willcox et al. (1973), respectively. This latter matrix was then used as the basis for an identification service in our laboratory, the methods for which were reviewed by Willcox et al. (1980). In the operation of the identification service, the test results obtained for strains submitted for identification were accumulated by computer. These results were then sorted by taxon and printed in the form of summaries, as described by Holmes & Hill (1985). From these summarized results a revised matrix was derived for the fermentative organisms and, following evaluation, this matrix is now in current use for the routine identification service.In this paper we present the results obtained in the identification of 933 strains of bacteria belonging to 110 taxa in the revised probability matrix. METHODS Overallprocedure.A matrix was constructed which gave the probability of a strain of any given taxon yielding a positive result in each of the chosen tests (Table 1). Individual strains were then identified on the basis of these results.Taxa. Of the taxa chosen for the matrix (Table I), the majority gave a fermentative result in the oxidation/fermentation (O/F test) of Hugh & Leifson (1953). A few non-fermentative taxa were also included; these were taxa that produce acid from glucose in peptone/water/sugar media and that may possess other characteristics by which they may be confused with fermentative organisms.Although the range of taxa was selected primarily to include those of known medical importance and those likely to occur in medical specimens, efforts were made to include as many recently described species as possible, so as to facilitate recognition of the latter should they occur in human clinical or veterinary material. Particular attention was paid to ensure inclusion of all Enterobacteriaceae taxa described in Bergeys Manual of Systematic Bacteriology (Brenner, 1984). The majority of the taxa are recognized species, genera or subgenera. Some are t Present address: Gibco-Sensititre, Imberhorne La...

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“…The 8th edition of Bergey's Manual of Determinative Bacteriology (23), following earlier work (10,11,20), recognizes only one species, Serratia marcescens. However, since 1959 several workers (1,12,14) have challenged the concept of a monospecific genus Serratia. The second species to be formally recognized was S. Ziquefaciens (Grimes and Hennerty 1931) Bascomb et al 1971 (1,13).…”

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confidence: 99%

“…However, since 1959 several workers (1,12,14) have challenged the concept of a monospecific genus Serratia. The second species to be formally recognized was S. Ziquefaciens (Grimes and Hennerty 1931) Bascomb et al 1971 (1,13). A third species, S. rubidaea (Stapp 1940) Ewing et al 1973, was proposed and corresponds to Serratia biotype 2 of Bascomb et al (1) and to phenon B of Grimont and Dulong de Rosnay (14).…”

mentioning

confidence: 99%

“…The second species to be formally recognized was S. Ziquefaciens (Grimes and Hennerty 1931) Bascomb et al 1971 (1,13). A third species, S. rubidaea (Stapp 1940) Ewing et al 1973, was proposed and corresponds to Serratia biotype 2 of Bascomb et al (1) and to phenon B of Grimont and Dulong de Rosnay (14). The same taxon is also called S. marinorubra ZoBell and Upham 1944.…”

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confidence: 99%

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Deoxyribonucleic Acid Relatedness Between Serratia plymuthica and Other Serratia Species, with a Description of Serratia odorifera sp. nov. (Type Strain: ICPB 3995)

Grimont

Richard

et al. 1978

International Journal of Systematic Bacteriology

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Seven new isolates of Serratiaplymuthica (including two from a human source) and 24 isolates resembling unclustered Serratia strain 38 (Grimont et al., J. Gen. Microbiol98:39-66, 1977) are described. Deoxyribonucleic acid relatedness studies, obtained with labeled reference deoxyribonucleic acid from s. plymuthica 392 confirm that S. plymuthica is a discrete species. Mean percent relatedness of S. plymuthica isolates to strain 392 was 88 f 13.6 (standard deviation), whereas the mean percent relatedness of S. Ziquefaciens isolates (the closest species) to strain 392 was 52 f 12.4. Strain 38 and twenty-four "38-like" isolates constitute a new deoxyribonucleic acid hybridization group that is 28 to 43% related to Serratia species and to the "Citrobacter-like" group of Leclerc and Buttiaux, and 15 to 22% related to other known species of Enterobacteriaceae. This 38-like group constitutes a new species that is named Serratia odorifera sp. nov. (type strain, ICPB 3995). Two biotypes (1 and 2) are described. Strains of this species will grow on caprylate-thallous agar (selective for Serratia spp.), and they have a characteristic odor. Most strains of S. odorifera were recovered from clinical specimens.

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Numerical Classification of the Tribe Klebsielleae

Cited by 118 publications

References 19 publications

Characterization of Serratia marcescens, S. liquefaciens, S. plymuthica and S. marinorubra by the Electrophoretic Patterns of their Esterases

Characterization of Serratia marcescens, S. liquefaciens, S. plymuthica and S. marinorubra by the Electrophoretic Patterns of their Esterases

A Revised Probability Matrix for the Identification of Gram-negative, Aerobic, Rod-shaped, Fermentative Bacteria

Deoxyribonucleic Acid Relatedness Between Serratia plymuthica and Other Serratia Species, with a Description of Serratia odorifera sp. nov. (Type Strain: ICPB 3995)

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