Transitional flow over cylindrical protuberances attached to a flat plate has been studied in a hypersonic wind tunnel. The tests were performed in the 31-Inch Mach 10 Air Tunnel at NASA Langley Research Center. Cylindrical protuberances with 4-mm diameter and heights of 1, 2, and 4 mm, were attached to the flat plate. The plate angle of attack and tunnel stagnation pressure were varied to achieve desired combinations of boundary layer edge Mach number and the boundary layer thickness. Several different types of flow visualization based on nitric oxide planar laser-induced fluorescence (NO PLIF) were used. A 10-Hz NO PLIF system was used to produce a thin laser sheet. This laser sheet was oriented perpendicular to the flat plate on some runs and parallel to the flat plate on others. A thick laser sheet was also used in some runs to provide visualization of entire flow structures inside the boundary layer. Viewing the PLIF intensity generated with the thick laser sheet using two cameras allowed stereoscopic visualization of the flow. In post processing, these images were combined to produce anaglyphs. Viewing these anaglyphs through blue and red 3D-glasses allows the three-dimensional flow structures to be observed. Another measurement system was a MHz-rate NO PLIF imaging system. Both PLIF measurement systems have better than one microsecond time resolution, which is fast enough to freeze the flow. The MHz system allows subsequent images to be obtained at one or two microsecond time intervals, resulting in movies of the flow. The data reported in this paper show the size, shape, and speed of flow structures in transitional hypersonic boundary layer flows and will provide valuable comparison data for computations of these flows.