Numerical Data Concerning the Sensitivity of Anaerobic Bacteria to Oxygen

Fredette, V.; Planté, C.; Roy, A.

doi:10.1128/jb.94.6.2012-2017.1967

Cited by 32 publications

(5 citation statements)

References 3 publications

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“…Assinala-se o aumento da zona de inibição, proporcionado em hiperoxia hiperbárica. Adaptado de FREDETTE et al (32) . Figura 3 -Hemoculturas quantitativas na peritonite fecal.…”

Section: Tensão De Oxigênio E Crescimento Microbianounclassified

Hiperoxia hiperbárica em infecções graves e sepse

Iazzetti

Mantovani

1998

Medicina (Ribeirão Preto)

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Efetua-se uma revisão da forma como a hiperoxigenação hiperbárica pode interferir em alguns dos fatores do quadro séptico, em especial na sepse provocada por infecções intra-abdominais, envolvendo bactérias Gram-negativas, destacando-se o seu interesse para a pesquisa experimental e clínica. São considerados alguns conceitos de hipoxia e metabolismo oxidativo na sepse, revisando-se o papel dos radicais ativados do oxigênio e antioxidantes, nesta síndrome. Conceituam-se as bases do uso da hiperoxia hiperbárica em infecções graves e sua utilização clinica atual.

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“…Assinala-se o aumento da zona de inibição, proporcionado em hiperoxia hiperbárica. Adaptado de FREDETTE et al (32) . Figura 3 -Hemoculturas quantitativas na peritonite fecal.…”

Section: Tensão De Oxigênio E Crescimento Microbianounclassified

Hiperoxia hiperbárica em infecções graves e sepse

Iazzetti

Mantovani

1998

Medicina (Ribeirão Preto)

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“…Anaerobic bacteria differ in their ability to survive in the presence of oxygen (7,21,29,32,33). It has been proposed that differences in oxygen tolerance among anaerobes may be related to the effectiveness of defense mechanisms possessed by bacteria against toxic products of oxygen reduction (24).…”

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confidence: 99%

Factors Related to the Oxygen Tolerance of Anaerobic Bacteria

Rolfe

Hentges

Campbell

et al. 1978

Appl Environ Microbiol

135

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The effect of atmospheric oxygen on the viability of 13 strains of anaerobic bacteria, two strains of facultative bacteria, and one aerobic organism was examined. There were great variations in oxygen tolerance among the bacteria. All facultative bacteria survived more than 72 h of exposure to atmospheric oxygen. The survival time for anaerobes ranged from less than 45 min for Peptostreptococcus anaerobius to more than 72 h for two Clostridium perfringens strains. An effort was made to relate the degree of oxygen tolerance to the activities of superoxide dismutase, catalase, and peroxidases in cell-free extracts of the bacteria. All facultative bacteria and a number of anaerobic bacteria possessed superoxide dismutase. There was a correlation between superoxide dismutase activity and oxygen tolerance, but there were notable exceptions. Polyacrylamide gel electropherograms stained for superoxide dismutase indicated that many of the anaerobic bacteria contained at least two electrophoretically distinct enzymes with superoxide dismutase activity. All facultative bacteria contained peroxidase, whereas none of the anaerobic bacteria possessed measurable amounts of this enzyme. Catalase activity was variable among the bacteria and showed no relationship to oxygen tolerance. The ability of the bacteria to reduce oxygen was also examined and related to enzyme content and oxygen tolerance. In general, organisms that survived for relatively long periods of time in the presence of oxygen but demonstrated little superoxide dismutase activity reduced little oxygen. The effects of medium composition and conditions of growth were examined for their influence on the level of the three enzymes. Bacteria grown on the surface of an enriched blood agar medium generally had more enzyme activity than bacteria grown in a liquid medium. The data indicate that superoxide dismutase activity and oxygen reduction rates are important determinants related to the tolerance of anaerobic bacteria to oxygen.

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“…Serum was added to the medium to determine whether it could protect against possible toxic effects of oxygen (6,9). The results correlate well with the requirement for hemin, displayed only by B. melaninogenicus, since it has been suggested that hemin has a neutralizing effect on oxygen toxicity (7).…”

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confidence: 81%

Growth of Bacteroidaceae in Stirred Fermentors

Wahren

Holme

1969

Applied Microbiology

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The conditions for increasing bacterial yields in cultures of Bacteroidaceae by the use of stirred fermentors and pH control were investigated by means of three representative species: Sphaerophorus necrophorus, Bacteroides fragilis, and B. melaninogenicus. A medium containing tryptone, yeast extract, and glucose or sucrose was used. Horse serum had to be added to obtain substantial growth of B. melaninogenicus. The optimal pH for growth rate and yield was 7.0 to 7.2. Lysis of the bacteria occurred when the glucose (or sucrose) was exhausted. The rate of lysis was very high in cultures of S. necrophorus, less so in B. fragilis and B. melaninogenicus. Pleomorphism, manifested as large spherical forms of the bacteria, was observed in the late logarithmic phase of S. necrophorus. Great differences in the length of the lag phase and of the mean generation time were found among the three bacterial species. The yield in static cultures of the three species without pH control was approximately 0.4 g of dry cells per liter, but was increased, in stirred fermentors with pH control, to 3.5 g (S. necrophorus), 2.7 g (B. fragilis), and 4.3 g (B. melaninogenicus) per liter. With an inoculum density of 5 to 10 mg (dry weight) per liter, these yields were obtained in approximately 10 (S. necrophorus), 25 (B. fragilis), and 35 hr (B. melaninogenicus), respectively.

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Numerical Data Concerning the Sensitivity of Anaerobic Bacteria to Oxygen

Cited by 32 publications

References 3 publications

Hiperoxia hiperbárica em infecções graves e sepse

Hiperoxia hiperbárica em infecções graves e sepse

Factors Related to the Oxygen Tolerance of Anaerobic Bacteria

Growth of Bacteroidaceae in Stirred Fermentors

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