“…The adsorption isotherm at equilibrium between the amounts adsorbed in the stationary phase and the concentration in the mobile phase determines the direction of the band asymmetry, either as a fronting or tailing shape. The overload band profiles and related the adsorption isotherms in reverse phase liquid chromatography (RP-LC) conditions using packed [8,9] and monolithic [10,11] columns have been studied to understand of retention and overloading mechanisms.…”