Numerical investigation into thermal effects of pre-cooling zone in vitrification-based cryopreservation process

Tsai, Hsun Heng; Tsai, Chien Hsiung; Wu, Wei; Chen, Fu Zen; Chiang, Pei Ju

doi:10.1016/j.cryobiol.2014.11.003

Cited by 8 publications

(3 citation statements)

References 20 publications

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“…Respiratory intensity was determined using the method of Tsai et al [22]. In brief, 100 g of strawberry samples were placed in a desiccator, together with a Petri dish containing 20 mL of 0.4 M NaOH solution.…”

Section: Respiratory Intensitymentioning

confidence: 99%

Effects of Litsea cubeba Essential Oil–Chitosan/Corn Starch Composite Films on the Quality and Shelf-Life of Strawberry (Fragaria × ananassa)

Fu,

Wang,

et al. 2024

Foods

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In this work, we have developed a composite chitosan film incorporating the Litsea cubeba essential oil (LCEO) and starch with good physical properties, and investigated the effect of coating strawberries with this composite film. The best formula of the LCEO/chitosan/corn starch/glycerol (LCEO/CH/CS/gly) composite films is 0.25% LCEO, 2.75% CH, 0.40% corn starch, and 0.75% glycerol. Coating strawberries with CH/CS/gly film or LCEO/CH/CS/gly films resulted in significantly lower respiration intensity and a slower decay rate, much slower decreases in the firmness, and reductions in the sugar and ascorbic acid content of the fruit during storage (p < 0.05). The coatings also led to a much slower accumulation of malondialdehyde and anthocyanins (p < 0.05). The LCEO/CH/CS/gly film was generally more effective than the CH/CS/gly film; however, the effect was more obvious in the later stages of storage. Thus, coating strawberries with CH/CS/gly film or LCEO/CH/CS/gly film can be a viable method for extending the shelf-life of the fruit.

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Section: Respiratory Intensitymentioning

confidence: 99%

Effects of Litsea cubeba Essential Oil–Chitosan/Corn Starch Composite Films on the Quality and Shelf-Life of Strawberry (Fragaria × ananassa)

Fu,

Wang,

et al. 2024

Foods

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“…The use of vitrification of fish spermatozoa is a relatively new application, however, it has been already tested on sperm of several fish species [66]. The success of sperm vitrification depends on several factors, including initial sperm quality, type and concentration of cryoprotectants, equilibration time and cooling and warming rates [67]. Normally, the concentration of cryoprotectants used must be very high to prevent the formation of ice crystals during the fast cooling process, but can be toxic to the cells.…”

Section: Vitrificationmentioning

confidence: 99%

Eel sperm cryopreservation: An overview

et al. 2019

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The eels are teleost fishes from the order Anguilliformes that includes several species with high commercial value. Due to the high interest for aquaculture production of some eel species and for the need to restore eel species that are endangered, several research groups have directed their research toward developing protocols to cryopreserve the spermatozoa of Japanese eel (Anguilla japonica) and European eel (Anguilla anguilla).In this review, we provide an overview on the different protocols that have been developed so far. The first developed protocols used DMSO as cryoprotectant in both species with good success, obtaining sperm motilities of over 45% in Japanese eel and over 35% in European eel. Moreover, sperm cryopreserved using DMSO was successfully used in fertilization trials, although with low fertilization rates. However, recent studies show that DMSO produce epigenetic changes in eel sperm and therefore, the last developed protocols used methanol as cryoprotectant instead. Cryopreservation protocols using methanol as cryoprotectant, showed improved motility values in both Japanese and European eel. In addition, the latest protocols have been adapted to cryopreserve larger volumes of sperm of up to 5 mL, which is useful for larger scale fertilization trials.The present study introduces the state of the art and future perspectives of the eel sperm cryopreservation to be applied in aquaculture and biological conservation programs.

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“…The use of vitrification of fish spermatozoa is a relatively new application, however, it has been already tested on sperm of several fish species (Xin et al, 2017). The success of sperm vitrification depends on several factors, including initial sperm quality, type and concentration of cryoprotectants, equilibration time and cooling and warming rates (Tsai et al, 2015). Normally, the concentration of cryoprotectants used must be very high to prevent the formation of ice crystals during the fast cooling process, but can be toxic to the cells.…”

Section: Vitrificationmentioning

confidence: 99%

Improvement of techniques for sperm evaluation and cryobanking in European eel.

Jusdado¹,

Germán²

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Numerical investigation into thermal effects of pre-cooling zone in vitrification-based cryopreservation process

Cited by 8 publications

References 20 publications

Effects of Litsea cubeba Essential Oil–Chitosan/Corn Starch Composite Films on the Quality and Shelf-Life of Strawberry (Fragaria × ananassa)

Effects of Litsea cubeba Essential Oil–Chitosan/Corn Starch Composite Films on the Quality and Shelf-Life of Strawberry (Fragaria × ananassa)

Eel sperm cryopreservation: An overview

Improvement of techniques for sperm evaluation and cryobanking in European eel.

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