This investigation into the aerodynamic efficiency and structural integrity of tubercle leading edges, inspired by the agile maneuverability of humpback whales, employs a multifaceted experimental and computational approach. By utilizing static load extensometer testing complemented by computational simulations, this study quantitatively assesses the impacts of unique wing geometries on aerodynamic forces and structural behavior. The experimental setup, involving a Wheatstone full-bridge circuit, measures the strain responses of tubercle-configured leading edges under static loads. These measured strains are converted into stress values through Hooke’s law, revealing a consistent linear relationship between the applied loads and induced strains, thereby validating the structural robustness. The experimental results indicate a linear strain increase with load application, demonstrating strain values ranging from 65 με under a load of 584 g to 249 με under a load of 2122 g. These findings confirm the structural integrity of the designs across varying load conditions. Discrepancies noted between the experimental data and simulation outputs, however, underscore the effects of 3D printing imperfections on the structural analysis. Despite these manufacturing challenges, the results endorse the tubercle leading edges’ capacity to enhance aerodynamic performance and structural resilience. This study enriches the understanding of bio-inspired aerodynamic designs and supports their potential in practical fluid mechanics applications, suggesting directions for future research on manufacturing optimizations.