Impellers are utilized to increase pressure to ensure that a radial pre-swirl system can provide sufficient cooling airflow to the turbine blades. In the open literature, the pressurization mechanism of the impellers was investigated. However, the effect of impellers on the cooling performance of the radial pre-swirl system was not clear. To solve the aforementioned problem, tests were carried out to assess the temperature drop in a radial pre-swirl system with various impeller configurations (impeller lengths l/b ranging from 0 to 0.333). Furthermore, numerical simulations were used to investigate the flow and heat transfer characteristics of the radial pre-swirl system at high rotating Reynolds numbers. Theoretical and experimental investigations revealed that the pre-swirl jet and output power generate a significant temperature drop, but the impellers have no obvious effect on the system temperature drop. By increasing the swirl ratio, the impellers reduce the field synergy angle and thus improve convective heat transfer on the turbine disk. In addition, increasing the impeller length can reduce the volume-averaged field synergy angle and improve heat transfer, but the improvement effectiveness decreases as the impeller length increases. Thus, the study concluded that impellers could improve the cooling performance of the radial pre-swirl system by enhancing disk cooling.