This paper aims to study the effects of activation energy and thermal radiation in the bioconvection flow of nanofluid (third-grade nanofluid) containing swimming microorganisms in the presence of a heat source-sink past a stretching sheet. Brownian movement and thermophoresis diffusion are used in mathematical modeling. The given flow phenomenon is modeled in the form of governing partial differential equations. Furthermore, appropriate dimensionless transformation is used to transfer the governing system of PDEs into an ordinary one. The remodeled systems of ODEs are tackled numerically by bvp4c on Matlab with a shooting scheme in computational tool MATLAB. The bearing of prominently involved parameters on the numerical solution of velocity, temperature distribution, nanoparticles concentration and concentration of microorganisms is comprehensively discussed and elaborated through figures. It is established that velocity can be improved with a mixed convection aspect. Furthermore, the temperature and concentration of nanoparticles reduce against Prandtl number, also, large Peclet number declines the microorganisms field. The work contained in this paper has applications in nanotechnology, electrical and mechanical engineering, biomedicine, biotechnology, drug delivery, cancer treatment, food processing and various industries. No such work is yet reported, and it is good for the research in applied sciences.