example which we have been dealing with is in vivo excitation of fluorescent proteins and specifically enhanced yellow fluorescent protein (eYFP) which has an emission cross section that is about 1.4 times higher than enhanced green fluorescent protein (eGFP) at its emission peak [1]. The goal is sufficient excitation of eYFP or the similar derivative Venus YFP [2] in order to obtain substantial stimulated emission in the environment of a living cell. These socalled living lasers have been demonstrated by employing eGFP as a molecule providing stimulated emission [3,4]. The peak excitation wavelength for the YFP family proteins is at 515 nm, accessible directly by frequency-doubled Yblasers. Continuous-wave or high-repetition-rate excitation is ruled out due to thermal cell damage and/or bleaching of the chromophore. Stable laser properties are necessary for such applications in order to maintain constant frequency conversion efficiency and a constant beam size in the excitation volume. Achieving this requires maintaining unchanged thermal conditions in the laser throughout the repetition rate tuning range. At the same time, the output wavelength has to be locked and the spectrum sufficiently narrows in order to maintain constant second harmonic generation (SHG) efficiency as the repetition rate is tuned.Here we target repetition rates from a few Hz to about 3 kHz, the upper bound limited by the radiative lifetime of the Yb 3+ excited state in Yb:KYW, and the laser material used in this work [5].There are several ways to achieve a pulsed laser source with controllable repetition rate. A seemingly simple solution for the tunable repetition rate source with stable output beam properties is an Yb-fiber amplifier seeded by an appropriate laser diode [6]. However, it is far from simple or inexpensive considering the need to effectively suppress amplified spontaneous emission and stabilize the output polarization, and it requires a laser diode which is Abstract We demonstrate a compact wavelength-stabilized, frequency-doubled Yb-doped double-tungstate laser with widely tunable repetition rate, spanning from 35 Hz to 3 kHz obtained by hybrid Q-switching. The Q-switching unit consisted of a combination of a passive Cr:YAG crystal and an opto-mechanical active intensity modulator. The fundamental wavelength was locked at 1029 nm with a volume Bragg grating, and the pulse length and energy were 42 ns and 250 µJ, respectively. As the laser was stabilized with the VBG and the opto-mechanical modulator, the frequency instability was reduced six times from free running down to 0.29 %. Frequency doubling was done extra-cavity in PPKTP, and a repetition rate-independent conversion efficiency of 63 % was obtained. The controllable repetition rate together with stable temporal and spatial characteristics makes this laser a suitable candidate in many biology-related experiments, as a pump source for in vivo excitation of fluorophores, e.g., pumping of "living lasers" and matrix-assisted laser desorption/ionization mass spectroscopy.