The main objective of this study is to understand the influence of various chemical reactions that participate on NO creation or reduction in N2/O2 mixed gas induced by negative corona discharge under different O2 concentrations (5%, 10%, 15%, 20% and 25%). The basic chemistry of NO evolution that is presented in this study is based on a comprehensive collection of processes that were gathered into 150 specific chemical reactions involving 25 molecular, excited, atomic, and charged entities. Without the diffusion and convective factors, the density was computed using the continuity equation over a range of electric reduction fields between 50 and 90 Td (1Td = 10−21 V.m2), at different points in the ranges 10−9–10−4 s. The outcomes of our numerical simulations demonstrate the impact of various chemical processes on NO production and decrease, including: N(2D) + O2 → NO + O and: NO + O + N2 → NO2 + N2 respectively. Our research has shown that at 50 and 70 Td, nitrogen oxide generation is dominated by an O2 concentration of 5%, whereas at 90 Td, it is dominated by an O2 concentration of 10%. These outcomes are true for both reactions.