Numerical simulation of critical particle size in asymmetrical deterministic lateral displacement

Rezaei, Behnam; Zand, Mahdi Moghimi; Javidi, Reza

doi:10.1016/j.chroma.2021.462216

Cited by 18 publications

(6 citation statements)

References 48 publications

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“…• Sorting efficiency highly dependent on the geometry and precise dimensions of the pillar array [ 109,262 ]…”

Section: Methods For Cell Sorting and Isolation In Integrated Microfl...mentioning

confidence: 99%

“…• Label-free separation [261] • Continuous sample processing possible [261,262] • Large size separation range (nanometer-to millimeter-sized objects) [110,262] • Separation principles not completely understood [262] • Sorting efficiency highly dependent on the geometry and precise dimensions of the pillar array [109,262] • Combination with DEP for enhanced separation efficiency [192] Microwells, microchambers and microtraps…”

Section: Inertial and Hydrodynamic Focusingmentioning

confidence: 99%

“…Cells/particles with radii larger than the critical radius migrate laterally to adjacent streamlines determined by the shape and the geometry of the micropillar array (Figure 4a). [11,108,109] In this manner, continuous sorting of cell populations, organelles, or particles is possible (Figure 4b). Significant advantages of DLD-based sorting include a high resolution (a separation limit down to 20 nm) and a high dynamic size-separation range, which spans from millimeter-to nanometer-sized objects.…”

Section: Microarchitecture-based Separation -Micropillars and Determi...mentioning

confidence: 99%

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Integrated Microfluidics for Single‐Cell Separation and On‐Chip Analysis: Novel Applications and Recent Advances

Kutluk,

Viefhues,

Constantinou

2024

Small Science

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From deciphering infection and disease mechanisms to identifying novel biomarkers and personalizing treatments, the characteristics of individual cells can provide significant insights into a variety of biological processes and facilitate decision‐making in biomedical environments. Conventional single‐cell analysis methods are limited in terms of cost, contamination risks, sample volumes, analysis times, throughput, sensitivity, and selectivity. Although microfluidic approaches have been suggested as a low‐cost, information‐rich, and high‐throughput alternative to conventional single‐cell isolation and analysis methods, limitations such as necessary off‐chip sample pre‐ and post‐processing as well as systems designed for individual workflows have restricted their applications. In this review, a comprehensive overview of recent advances in integrated microfluidics for single‐cell isolation and on‐chip analysis in three prominent application domains are provided: investigation of somatic cells (particularly cancer and immune cells), stem cells, and microorganisms. Also, the use of conventional cell separation methods (e.g., dielectrophoresis) in unconventional or novel ways, which can advance the integration of multiple workflows in microfluidic systems, is discussed. Finally, a critical discussion related to current limitations of integrated microfluidic single‐cell workflows and how they could be overcome is provided.

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“…• Sorting efficiency highly dependent on the geometry and precise dimensions of the pillar array [ 109,262 ]…”

Section: Methods For Cell Sorting and Isolation In Integrated Microfl...mentioning

confidence: 99%

Section: Inertial and Hydrodynamic Focusingmentioning

confidence: 99%

Section: Microarchitecture-based Separation -Micropillars and Determi...mentioning

confidence: 99%

See 1 more Smart Citation

Integrated Microfluidics for Single‐Cell Separation and On‐Chip Analysis: Novel Applications and Recent Advances

Kutluk,

Viefhues,

Constantinou

2024

Small Science

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“…The ow in the DLD domain can be modelled using the 2D Navier-Stokes equation (eqn (1)). 7,35 The ow depends on the value of the dimensionless numbers, such as Reynolds' number given in eqn (2). This dimensionless number depends on the density, velocity, dynamic viscosity and hydraulic diameter.…”

Section: Fabrication Of Pdms Devicesmentioning

confidence: 99%

Design and fabrication of a polydimethylsiloxane device for evaluating the effect of pillar geometry and configuration in the flow separation using deterministic lateral displacement

Pandit,

Kong,

Samuel

2024

RSC Adv.

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“…DLD is a size-based microfluidic technique making use of a pillar gradient array with a special critical size for EV separation. [155,156] The flow path of EVs whose size is larger than the critical criterion will be altered, while the other EVs follow the initial streamline. Our group developed a DLD platform with pillar shape designed based on topology optimization (TO) and a wide DLD channel, which altered zigzag mode to manipulate particles for the first time (Figure 4a).…”

Section: Passive Ev Isolationmentioning

confidence: 99%

From Conventional to Microfluidic: Progress in Extracellular Vesicle Separation and Individual Characterization

Chen

Lin

Cheng

et al. 2023

Adv Healthcare Materials

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Extracellular vesicles (EVs) are nanoscale membrane vesicles, which contain a wide variety of cargo such as proteins, miRNAs, and lipids. A growing body of evidence suggests that EVs are promising biomarkers for disease diagnosis and therapeutic strategies. Although the excellent clinical value, their use in personalized healthcare practice is not yet feasible due to their highly heterogeneous nature. Taking the difficulty of isolation and the small size of EVs into account, the characterization of EVs at a single‐particle level is both imperative and challenging. In a bid to address this critical point, more research has been directed into a microfluidic platform because of its inherent advantages in sensitivity, specificity, and throughput. This review discusses the biogenesis and heterogeneity of EVs and takes a broad view of state‐of‐the‐art advances in microfluidics‐based EV research, including not only EV separation, but also the single EV characterization of biophysical detection and biochemical analysis. To highlight the advantages of microfluidic techniques, conventional technologies are included for comparison. The current status of artificial intelligence (AI) for single EV characterization is then presented. Furthermore, the challenges and prospects of microfluidics and its combination with AI applications in single EV characterization are also discussed. In the foreseeable future, recent breakthroughs in microfluidic platforms are expected to pave the way for single EV analysis and improve applications for precision medicine.

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Numerical simulation of critical particle size in asymmetrical deterministic lateral displacement

Cited by 18 publications

References 48 publications

Integrated Microfluidics for Single‐Cell Separation and On‐Chip Analysis: Novel Applications and Recent Advances

Integrated Microfluidics for Single‐Cell Separation and On‐Chip Analysis: Novel Applications and Recent Advances

Design and fabrication of a polydimethylsiloxane device for evaluating the effect of pillar geometry and configuration in the flow separation using deterministic lateral displacement

From Conventional to Microfluidic: Progress in Extracellular Vesicle Separation and Individual Characterization

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