A miniaturized biosensor capable of detecting multiple analytes is proposed with real-time measuring and high sensitivity which are crucial for future sensing devices. We introduce here a dual-channel multi-analyte single polarization (DCSP) photonic crystal fiber (PCF) sensor. The DCSP sensor was fabricated using the stack-and-draw technique to show the sensor practical feasibility. The DCSP offers maximum wavelength and amplitude sensitivities of 11,000 nm/RIU and 807 RIU -1 , respectively in both channels for the analyte refractive index (RI) of na=1.33 to 1.41. The wavelength sensitivities are improved in this case by using (DCSP) sensors, which aid in the detection of multi-analyte, high-sensitivity, and real-time measurements. The DCSP sensor can be a suitable candidate for biosensing where the sample can be in a micro dimension due to having high sensing response. Moreover, the sensor works only at single polarization with lower confinement loss and better wavelength resolution, enhancing the previous works. These results indicate the potential use of the proposed DCSP sensors to improve future sensing technology and sensor's performance in the detection of diseases diagnostic and liquid detection and so on.