Numerical Simulations of Thermocapillary Flow of a Binary Mixture with the Soret Effect in a Shallow Annular Pool

Yu, Jiajia; Zhang, Li; Li, You–Rong; Chen, Jie-Chao

doi:10.1007/s12217-015-9485-2

Cited by 11 publications

(8 citation statements)

References 27 publications

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“…Depending on the density of the cell culture and the density of the surrounding medium, the speed to be applied may vary greatly. 2, 24 Due to the technical limitations an adaption with respect of the lager diameter of the vessel of the PMT device was not applicable. In order to evaluate this system with respect to potential shear forces further experiments need an configuration change of the photon collecting aperture to measure all emitted photons over the larger diameter.…”

Section: Discussionmentioning

confidence: 99%

“…To take this problem into account, online fixation and kinetic recording are important to improve the quality of simulation. 2 New methods such as ‘‘omics’’ technologies demand large quantities of exposed cell material. Multi-generation experiments need optimal cultivation conditions and medium changes, which can hardly be achieved during rotation of a cell culture in suspension in a small volume.…”

Section: Discussionmentioning

confidence: 99%

“…Recent experimental set-ups allow online kinetics during exposure by coupling of the clinostat or a RPM with PMTs or online observation by combination with microscopy. 2, 27, 28 Thereby, new results are added to the discussion about induced non-gravitational effects. They enable us to visualize the localization and behavior of cells, whether they are kept in stable positions or whether they are shifted and drifted during operation of the rotating device.…”

Section: Discussionmentioning

confidence: 99%

“…Under optimal conditions, the diameter of the containers is kept small (in the range of a few mm) and the objects are placed in the center of rotation in order to keep accelerations as minimal as possible. 2, 4 Zero head space and complete filling of the sample containers are essential to decrease mechanical disturbances. A 2D clinostat is constantly operated in one direction thereby inducing a static change of the gravity vector in relation to the rotated sample.…”

Section: Introductionmentioning

confidence: 99%

“…5–7 The mode of operation is commonly used for the terminology: while 3D clinostats are continuously rotating by changing the rate of rotation of the two motors at random, 5 a RPM is characterized by not only changing the velocity but additionally the direction of rotation in a real random mode. 2, 3 …”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Pyrocystis noctiluca represents an excellent bioassay for shear forces induced in ground-based microgravity simulators (clinostat and random positioning machine)

2017

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Ground-based facilities, such as clinostats and random positioning machines aiming at simulating microgravity conditions, are tools to prepare space experiments and identify gravity-related signaling pathways. A prerequisite is that the facilities are operated in an appropriate manner and potentially induced non-gravitational effects, such as shearing forces, have to be taken into account. Dinoflagellates, here P. noctiluca, as fast and sensitive reporter system for shear stress and hydrodynamic gradients, were exposed on a clinostat (constant rotation around one axis, 60 rpm) or in a random positioning machine, that means rotating around two axes, whose velocity and direction were chosen at random. Deformation of the cell membrane of P. noctiluca due to shear stress results in a detectable bioluminescence emission. Our results show that the amount of mechanical stress is higher on an random positioning machine than during constant clinorotation, as revealed by the differences in photon counts. We conclude that one axis clinorotation induced negligible non-gravitational effects in the form of shear forces in contrast to random operation modes tested. For the first time, we clearly visualized the device-dependent occurrence of shear forces by means of a bioassay, which have to be considered during the definition of an appropriate simulation approach and to avoid misinterpretation of results.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Pyrocystis noctiluca represents an excellent bioassay for shear forces induced in ground-based microgravity simulators (clinostat and random positioning machine)

2017

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Thermocapillary Convection in a Binary Mixture with Moderate Prandtl Number in a Shallow Annular Pool

Zhang

Luo

et al. 2017

Microgravity Sci. Technol.

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Effects of Non-Uniform Temperature of the Walls on the Soret Experiment

Lyubimova

Zubova

Shevtsova

2018

Microgravity Sci. Technol.

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We present a numerical study of a Soret experiment, which is conducted in a cell filled with a binary mixture and placed between differently heated horizontal walls. In real experiments, in the corner regions of the cell, where glass is in contact with metal, the uniformity of temperature can be perturbed. We study the effect of the temperature non-uniformity on the Soret separation considering two variable parameters: the local deviation of temperature from the value on the rest of the wall, and the length of the interval on which temperature perturbations occur. The cases of symmetric and non-symmetric perturbations are examined. Our non-linear numerical simulations reveal two distinct regimes of mass transfer in a mixture with Soret effect: diffusive and convective. The diffusive regime is sustained until temperature non-uniformity less than 3-5% of the applied temperature difference and occupies 5% of the wall length at each corner. In the convective regime, the emerging flow prohibits component separation by mixing of liquid components. It is noted a strong flow appears at the earliest moment of the experiment which rapidly attenuates.

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Numerical Simulations of Thermocapillary Flow of a Binary Mixture with the Soret Effect in a Shallow Annular Pool

Cited by 11 publications

References 27 publications

Pyrocystis noctiluca represents an excellent bioassay for shear forces induced in ground-based microgravity simulators (clinostat and random positioning machine)

Pyrocystis noctiluca represents an excellent bioassay for shear forces induced in ground-based microgravity simulators (clinostat and random positioning machine)

Thermocapillary Convection in a Binary Mixture with Moderate Prandtl Number in a Shallow Annular Pool

Effects of Non-Uniform Temperature of the Walls on the Soret Experiment

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