This paper concentrates on the error estimation of novel time second-order splitting conservative finite difference method (FDM) for high-dimensional nonlinear fractional Schrödinger equation rigorously. The discrete preservation property of our scheme is exhibited. By virtue of the cut-off technique and discrete energy method, it is shown that our scheme possesses the accuracy of (Δt 2 + h 2x + h 2 𝑦 ) in sense of L 2 -norm. Numerical experiments are exhibited to validate the accuracy and conservation property of our scheme.