In this paper, a combined theoretical and experimental study is carried out to investigate the spanwise effect of turbulence on the aerodynamic lift on a wing with different aspect ratios. The ratio of the mean square variance of the aerodynamic lift calculated by the commonly used strip theory and the two-wavenumber buffeting theory is analyzed comprehensively for the wings with different aspect ratios in turbulence with various integral scales. To validate the theoretical analysis and achieve a deeper understanding of the spanwise effects of turbulence, wind tunnel experiments are performed on National Advisory Committee for Aeronautics 0015 airfoils in grid-generated turbulent flows with different integral scales. The results demonstrate that it is essential to use the two-wavenumber buffeting theory to account for the spanwise effect of turbulence when calculating the aerodynamic lifts on wings with small aspect ratios, especially when in small-scale turbulence. The deviations between the equivalent two-wavenumber coherence function and the spanwise effect influence function at low reduced streamwise wavenumbers are the underlying causes for spanwise effects of turbulence. To achieve reliable wind tunnel testing results, appropriate simulations of the ratio of the turbulence integral scale to the chord are very important in the measurements of aerodynamic lifts on finite-span wing sections, especially for those with small aspect ratios.