The purpose of this paper is to develop a numerical scheme for the two-dimensional fourth-order fractional subdiffusion equation with variable coefficients and delay. Using the L2−1σ approximation of the time Caputo derivative, a finite difference method with second-order accuracy in the temporal direction is achieved. The novelty of this paper is to introduce a numerical scheme for the problem under consideration with variable coefficients, nonlinear source term, and delay time constant. The numerical results show that the global convergence orders for spatial and time dimensions are approximately fourth order in space and second-order in time.