Aim: This study aimed to elucidate experience-related differences in the impact of an intervention program promoting ethical nursing practices among pediatric nurses with 0-5 years' experience in varying settings. Methods: The pediatric nursing care model, defining 24 elements of pediatric nursing practice, was developed to educate nurses with pediatric nursing experience only, as well as those with experience in other settings, on ethical nursing practices toward pediatric patients and their families. Participants' level of knowledge and frequency of application of these practices were assessed before, directly after and two months after the intervention, with a final follow-up three months post-intervention. Results: Results showed that among those who have experience in nursing areas other than pediatrics, the frequency of practicing items related to children's rights and dignity significantly increased (p < 0.05). Among nurses who have experience only in pediatric nursing, improvement was observed in their practice, including details important in building a relationship of trust between patients and health professionals, information disclosure and supportive care (p < 0.05). Conclusions: Participants showed an increased awareness of issues related to ethical pediatric nursing practice for patients' and their parents.