Introduction: A care bundle is a collection of evidence-based interventions that have been proved to improve patient care and clinical outcomes significantly. Aim of the study was to evaluate the effect of implementing a bundle of care for peripheral intravenous catheter on nurses' performance at the pediatric intensive care unit. Quasi experimental research design was used. Subjects and Method: All nurses (60) who were responsible for providing care for children who received peripheral venous catheter infusion or medication (Pediatric Intensive Care Unit of Tanta Main University Hospital. Also, sixty pediatric patients who had peripheral venous catheters were included 30 children before, immediately after, and 30 children after one month from the care bundle implementation. Three tools were used to collect data: A structured interview schedule about peripheral Intravenous Catheter care bundle, an observation Checklist for Peripheral Intravenous Catheter Bundle of Care, and a Visual Infusion Phlebitis Grading Scale (V.I.P). Results: Revealed that the total scores of nurses' knowledge and practice for most of the participated nurses were improved immediately after and after one month. Also, according to visual Infusion phlebitis grading scales (VIP), phlebitis was encountered among more than three-fifths of studied children and there was a significant improvement immediately after and after one month. Conclusion: Significant enhancement was found in the mean scores of total levels of nurses' knowledge and practice immediately after and one month after implementing the peripheral intravenous care bundle. Total scores of the studied children's Visual Infusion Phlebitis grading scale were reduced post immediate implantation of care bundle and after one month when compared with pre-implementation of care bundle. Recommendation: Establish a competency checklist for nurses to evaluate their competency in implementing PVCs maintenance care bundle.