Nutraceutical properties and polyphenolic profile of berry skin and wine of Vitis vinifera L. (cv. Aglianico)

Nisco, Mauro De; Manfra, Michele; Bolognese, Adele; Sofo, Adriano; Scopa, Antonio; Tenore, Gian Carlo; Pagano, Francesco; Milite, Ciro; Russo, Maria Teresa

doi:10.1016/j.foodchem.2012.10.123

Cited by 63 publications

(41 citation statements)

References 22 publications

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“…In the present study, the antioxidant properties of grape extracts were evaluated by hydrogen peroxide (H 2 O 2 ), hydroxyl radical (OH•) and DPPH-scavenging assays. Studies have reported the free radical scavenging activity measured by DPPH assay and hydroxyl radicals and hydrogen peroxide scavenging by grape extracts [11,35]. In this study, grape skin showed higher scavenging activity compared to its pulp, and Flame seedless cultivar exhibited highest activity.…”

Section: Discussionmentioning

confidence: 57%

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Free Radical Scavenging Properties of Skin and Pulp Extracts of Different Grape Cultivars In Vitro and Attenuation of H2O2-Induced Oxidative Stress in Liver Tissue Ex Vivo

Singha

Das

2014

Ind J Clin Biochem

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Grapes are the richest source of antioxidants due to the presence of potent bioactive phytochemicals. In this study, the phytochemical contents, scavenging activities and protective role against H 2 O 2 -induced oxidative stress in liver tissue ex vivo of four grape (Vitis vinifera) cultivars extracts, namely Flame seedless (black), Kishmish chorni (black with reddish brown), Red globe (red) and Thompson seedless mutant (green), were evaluated. The total phenolics and flavonoids content in pulp or skin fractions of different grape cultivars were in the range of 47.6-310 mg gallic acid equivalent/g fresh weight (fw), and 46.6-733.3 lg catechin equivalent/g fw respectively. The scavenging activities in skin of different grape varieties against 2,2-diphenyl-1-picrylhydrazyl (44-58 %), hydrogen peroxide (15.3-18.6 %), and hydroxyl radicals (50-85 %), were higher than pulp of the corresponding cultivars. These scavenging activities of grape extracts were found to be significantly (p \ 0.01) correlated with the levels of total phenols, flavonoids and ascorbic acid. Liver tissues from goat treated with H 2 O 2 (500 lM) showed significantly decreased GSH content by 42.9 % and activities of catalase by 50 % and glutathione reductase by 66.6 %; while increased thiobarbituric acid reactive substances and nitric oxide level by 2.53-and 0.86-fold, respectively, and activity of glutathione S-transferase by 0.96-fold. Grape skin extracts showed the stronger protective activity against H 2 O 2 -induced oxidative stress in liver tissue ex vivo, than its pulp of any cultivar; and the Flame seedless (black) cultivar showed the highest potential. In conclusion, our study suggested that the higher antioxidant potential, phytochemical contents and significant scavenging capacities in pulp and skin of grape extracts showed the protective action of grape extracts against H 2 O 2 -induced oxidative stress in liver tissue ex vivo.

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Section: Discussionmentioning

confidence: 57%

“…These phytochemicals are responsible for nutraceutical and health benefits [11]. India grows approximately 20 different cultivars of grapes, under diverse soil and climatic conditions including the Thompson seedless being the most popular cultivar.…”

Section: Introductionmentioning

confidence: 99%

Free Radical Scavenging Properties of Skin and Pulp Extracts of Different Grape Cultivars In Vitro and Attenuation of H2O2-Induced Oxidative Stress in Liver Tissue Ex Vivo

Singha

Das

2014

Ind J Clin Biochem

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“…The nutraceutical properties of the grape berry and its derivatives, namely wine, are commonly associated with the antioxidant properties of the phenolic compounds they contain (Tenore et al, 2011;De Nisco et al, 2013), from simple flavonoids like anthocyanins to condensed proanthocyanidins (PAs, tannins), which can be solubilized into the vacuole or linked to cell wall polysaccharides, so, it is of great interest to understand their antioxidant mechanisms in planta.…”

Section: Introductionmentioning

confidence: 99%

Oxidative stress homeostasis in grapevine (Vitis vinifera L.)

Carvalho

Vidigal

Amâncio

2015

Front. Environ. Sci.

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Plants can maintain growth and reproductive success by sensing changes in the environment and reacting through mechanisms at molecular, cellular, physiological, and developmental levels. Each stress condition prompts a unique response although some overlap between the reactions to abiotic stress (drought, heat, cold, salt or high light) and to biotic stress (pathogens) does occur. A common feature in the response to all stresses is the onset of oxidative stress, through the production of reactive oxygen species (ROS). As hydrogen peroxide and superoxide are involved in stress signaling, a tight control in ROS homeostasis requires a delicate balance of systems involved in their generation and degradation. If the plant lacks the capacity to generate scavenging potential, this can ultimately lead to death. In grapevine, antioxidant homeostasis can be considered at whole plant levels and during the development cycle. The most striking example lies in berries and their derivatives, such as wine, with nutraceutical properties associated with their antioxidant capacity. Antioxidant homeostasis is tightly regulated in leaves, assuring a positive balance between photosynthesis and respiration, explaining the tolerance of many grapevine varieties to extreme environments. In this review we will focus on antioxidant metabolites, antioxidant enzymes, transcriptional regulation and cross-talk with hormones prompted by abiotic stress conditions. We will also discuss three situations that require specific homeostasis balance: biotic stress, the oxidative burst in berries at veraison and in vitro systems. The genetic plasticity of the antioxidant homeostasis response put in evidence by the different levels of tolerance to stress presented by grapevine varieties will be addressed. The gathered information is relevant to foster varietal adaptation to impending climate changes, to assist breeders in choosing the more adapted varieties and suitable viticulture practices.

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“…[25][26][27] HPLC-MS analysis was performed using an Agilent 1200 Series Rapid Resolution HPLC system coupled to a hybrid quadrupole TOF QSTAR Elite (Applied Biosystems/MDS Sciex) according to the protocol for untargeted metabolomic analysis established in our group. [25][26][27] Thus, chromatographic separation was performed using a RP 18 Luna 5 µm, 50 × 2.0 mm column (Phenomenex, Torrance, CA) in mobile phases consisted of (A) 0.1% HCOOH and (B) ACN 0.1% HCOOH, at a flow rate of 600 µL min −1 with the following linear gradient elution (t, %B): (0, 1), (4,20), (6,95), (7.5, 95), (8,1), (12,1). q-TOF spray parameters were set as previously described 27 and full data acquisition was performed in the scan range 70-700 m/z in positive ionization mode.…”

Section: Lc-ms Metabolomics Analysismentioning

confidence: 99%

“…4,5,8 Current progress in the development and implementation of omics' technologies has opened new perspectives in nutrition research, 9 e.g. food metabolome analysis, 10 and, consequently, they have become widely applied in nutraceutical investigation.…”

Section: Introductionmentioning

confidence: 99%

Metabolic fingerprint after acute and under sustained consumption of a functional beverage based on grape skin extract in healthy human subjects

et al. 2015

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Grape-derived polyphenols are considered to be one of the most promising ingredients for functional foods due to their health-promoting activities. We applied a HPLC-MS-based untargeted metabolomic approach in order to evaluate the impact of a functional food based on grape skin polyphenols on the urinary metabolome of healthy subjects. Thirty-one volunteers participated in two dietary crossover randomized intervention studies: with a single-dose intake (187 mL) and with a 15-day sustained consumption (twice per day, 187 mL per day in total) of a functional beverage (FB). Postprandial (4-hour) and 24-hour urine samples collected after acute consumption and on the last day of sustained FB consumption, respectively, were analysed using an untargeted HPLC-qTOF-MS approach. Multivariate modelling with subsequent application of an S-plot revealed differential mass features related to acute and prolonged consumption of FB. More than half of the mass features were shared between the two types of samples, among which several phase II metabolites of grape-derived polyphenols were identified at confidence level II. Prolonged consumption of FB was specifically reflected in urine metabolome by the presence of first-stage microbial metabolites of flavanols: hydroxyvaleric acid and hydroxyvalerolactone derivatives.Overall, several epicatechin and phenolic acid metabolites both of tissular and microbiota origin were the most representative markers of FB consumption. To our knowledge, this is one of the first studies where an untargeted LC-MS metabolomic approach has been applied in nutrition research on a grapederived FB.

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Nutraceutical properties and polyphenolic profile of berry skin and wine of Vitis vinifera L. (cv. Aglianico)

Cited by 63 publications

References 22 publications

Free Radical Scavenging Properties of Skin and Pulp Extracts of Different Grape Cultivars In Vitro and Attenuation of H2O2-Induced Oxidative Stress in Liver Tissue Ex Vivo

Free Radical Scavenging Properties of Skin and Pulp Extracts of Different Grape Cultivars In Vitro and Attenuation of H2O2-Induced Oxidative Stress in Liver Tissue Ex Vivo

Oxidative stress homeostasis in grapevine (Vitis vinifera L.)

Metabolic fingerprint after acute and under sustained consumption of a functional beverage based on grape skin extract in healthy human subjects

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