Nutrient Availability and Phage Exposure Alter the Quorum-Sensing and CRISPR-Cas-Controlled Population Dynamics of Pseudomonas aeruginosa

Ahator, Stephen Dela; Sagar, Sadhanna; Zhu, Minya; Wang, Jianhe; Zhang, Lian‐Hui

doi:10.1128/msystems.00092-22

Cited by 5 publications

(1 citation statement)

References 67 publications

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“…QS in P. aeruginosa consists of four known hierarchically organized systems, Las, Rhl, Pqs, and Iqs ( Fig. 2 ) [ [96] , [97] , [98] ]. Each of these systems contains a synthase to produce a signaling molecule known as AI and a receptor which is a transcriptional regulator.…”

Section: Quorum Sensing In P Aeruginosamentioning

confidence: 99%

Bile effects on the Pseudomonas aeruginosa pathogenesis in cystic fibrosis patients with gastroesophageal reflux

Bayat,

Nahand,

Farsad-Akhatr

et al. 2023

Heliyon

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Section: Quorum Sensing In P Aeruginosamentioning

confidence: 99%

Bile effects on the Pseudomonas aeruginosa pathogenesis in cystic fibrosis patients with gastroesophageal reflux

Bayat,

Nahand,

Farsad-Akhatr

et al. 2023

Heliyon

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Analysis of CRISPR–Cas loci distribution in Xanthomonas citri and its possible control by the quorum sensing system

Martins,

Granato,

Morgan

et al. 2024

FEMS Microbiology Letters

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Xanthomonas is an important genus of plant-associated bacteria that causes significant yield losses of economically important crops worldwide. Different approaches have assessed genetic diversity and evolutionary interrelationships among the Xanthomonas species. However, information from clustered regularly interspaced short palindromic repeats (CRISPR) has yet to be explored. In this work, we analyzed the architecture of CRISPR-Cas loci and presented a sequence similarity-based clustering of conserved Cas proteins in different species of Xanthomonas. Although absent in many investigated genomes, Xanthomonas harbors subtype I-C and I-F CRISPR-Cas systems. The most represented species, Xanthomonas citri, presents a great diversity of genome sequences with an uneven distribution of CRISPR-Cas systems among the subspecies/pathovars. Only Xanthomonas citri subsp. citri and Xanthomonas citri pv. punicae have these systems, exclusively of subtype I-C system. Moreover, the most likely targets of the X. citri CRISPR spacers are viruses (phages). At the same time, few are plasmids, indicating that CRISPR/Cas system is possibly a mechanism to control the invasion of foreign DNA. We also showed in X. citri susbp. citri that the cas genes are regulated by the diffusible signal factor (DSF), the quorum sensing (QS) signal molecule, according to cell density increases, and under environmental stress like starvation. These results suggest that the regulation of CRISPR-Cas by QS occurs to activate the gene expression only during phage infection or due to environmental stresses, avoiding a possible reduction in fitness. Although more studies are needed, CRISPR-Cas systems may have been selected in the Xanthomonas genus throughout evolution, according to the cost-benefit of protecting against biological threats and fitness maintenance in challenging conditions.

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Drivers and consequences of bacteriophage host range

Holtappels

Alfenas‐Zerbini

Koskella

2023

FEMS Microbiology Reviews

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Bacteriophages are obligate parasites of bacteria characterized by the breadth of hosts that they can infect. This ‘host range’ depends on the genotypes and morphologies of the phage and the bacterial host, but also on the environment in which they are interacting. Understanding phage host range is critical to predicting the impacts of these parasites in their natural host communities and their utility as therapeutic agents, but is also key to predicting how phages evolve and in doing so drive evolutionary change in their host populations, including through movement of genes among unrelated bacterial genomes. Here, we explore the drivers of phage infection and host range from the molecular underpinnings of the phage-host interaction to the ecological context in which they occur. We further evaluate the importance of intrinsic, transient, and environment drivers shaping phage infection and replication, and discuss how each influence host range over evolutionary time. The host range of phages has great consequences in phage-based application strategies, as well as natural community dynamics, and we therefore highlight both recent developments and key open questions in the field as phage-based therapeutics come back into focus.

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Nutrient Availability and Phage Exposure Alter the Quorum-Sensing and CRISPR-Cas-Controlled Population Dynamics of Pseudomonas aeruginosa

Abstract: The increase in antimicrobial resistance has created the need for alternative interventions such as phage therapy. However, as previously observed with antimicrobial resistance, phage therapy will not be effective if bacteria evolve resistance and persist in the presence of the phages.

Cited by 5 publications

References 67 publications

Bile effects on the Pseudomonas aeruginosa pathogenesis in cystic fibrosis patients with gastroesophageal reflux

Bile effects on the Pseudomonas aeruginosa pathogenesis in cystic fibrosis patients with gastroesophageal reflux

Analysis of CRISPR–Cas loci distribution in Xanthomonas citri and its possible control by the quorum sensing system

Drivers and consequences of bacteriophage host range

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