A high performance liquid chromatography (HPLC) method was developed for analyzing vitamin B1, and the method was used with other HPLC methods to monitor vitamins C, B1, and B2 of fresh fruits and vegetables stored at different temperatures. Ascorbic acid content of most commodities either dropped after being placed in storage or decreased during storage. Thiamine increased during storage of ‘Tendergreen’ beans and decreased in ‘BelRus’ potatoes; whereas riboflavin decreased in ‘Tendergreen’ beans and increased in ‘Superior’ potatoes. The changes in other cultivars or commodities were not significant.