The proportion of the biological nitrogen demand met by nitrate (f-ratio) is a strong candidate for a biogeochemical parameter to relate primary production to the oceanic organic carbon flux, the latter being a potential global carbon sink. There is a controversy over whether-Scan be related to spatial and temporal variations in NO,-concentration for the purposes of estimating carbon fluxes. I report measurements of NO,-and NH, t uptake rates and of the&ratio taken during a coastal phytoplankton bloom and address the problem of mesoscale f-ratio variability.The results reveal two distinct behaviors of the f-ratio depending on light level: at the 50% level,fis nonlinearly related to ambient NO, concentration; at the 10 and 1% light levels, fis not related to NO3 concentration but is instead related to variations in the stratification in the NO,-profile. There seems to be a "two-track" biological system in the euphotic zone: a shallow food web that quickly incorporates new NO,-supplies and a deep food web that responds more slowly to a varying supply. New production tends to be higher near the surface than deeper, which is opposite the vertical structure generally proposed for NO, -limited systems. The implication is that NO, --based algorithms to predict f may not work over the full three-dimensional NO, field.