Nutrient-induced signal transduction through the protein kinase A pathway and its role in the control of metabolism, stress resistance, and growth in yeast

Thevelein, Johan M.; Cauwenberg, Liesbet; Colombo, Sonia; Winde, Johannes H. de; Donation, Monica; Dumortier, Françoise; Kraakman, L.; Lemaire, Katleen; Packham, MA; Nauwelaers, David; Rolland, Filip; Teunissen, Aloys; Dijck, Patrick Van; Versele, Matthias; Wera, Stefaan; Winderickx, Joris

doi:10.1016/s0141-0229(00)00177-0

Cited by 128 publications

(117 citation statements)

References 43 publications

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“…Microorganisms are often exposed to a broad range of variations, especially regarding the quality and availability of nutrients, which influence the progression of their life cycle, alter physiological and morphological patterns, and may influence the profile of secreted proteins (Thevelein et al, 2000;Oliver et al, 2002;Medina et al, 2004;Vanoni et al, 2005;Bouws et al, 2008). In yeast, the correlation of nutrient deprivation and these responses is well described.…”

Section: Discussionmentioning

confidence: 99%

“…In yeast, the correlation of nutrient deprivation and these responses is well described. Usually, growth with GLU, or a related rapidly fermentable sugar, stimulates rapid growth and the development of larger cells (Thevelein et al, 2000;Vanoni et al, 2005). In the filamentous fungus M. perniciosa, rapid growth judged by biomass production was observed when mycelia grew in the presence of GLU or similar fermentable sugars, e.g., fructose and mannose, which enter the glycolytic pathway directly ( Figure 1B).…”

Section: Discussionmentioning

confidence: 99%

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Carbon source-induced changes in the physiology of the cacao pathogen Moniliophthora perniciosa (Basidiomycetes) affect mycelial morphology and secretion of necrosis-inducing proteins

Alvim¹,

Mattos²,

Pirovani³

et al. 2009

Genet. Mol. Res.

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ABSTRACT. Quantitative and qualitative relationships were found between secreted proteins and their activity, and the hyphal morphology of Moniliophthora perniciosa, the causal agent of witches' broom disease in Theobroma cacao. This fungus was grown on fermentable and non-fermentable carbon sources; significant differences in mycelial morphology were observed and correlated with the carbon source. A biological assay performed with Nicotiana tabacum leaves revealed that the necrosis-related activity of extracellular fungal proteins also differed with carbon source. There were clear differences in the type and quantity of the secreted proteins. In addition, the expression of the cacao molecular chaperone BiP increased after treatment with secreted proteins, suggesting a physiological response to the fungus secretome. We suggest that the carbon source-dependent energy metabolism of M. perniciosa results in physiological alterations in protein expression and secretion; these may affect not only M. perniciosa growth, but also its ability to express pathogenicity proteins.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Carbon source-induced changes in the physiology of the cacao pathogen Moniliophthora perniciosa (Basidiomycetes) affect mycelial morphology and secretion of necrosis-inducing proteins

Alvim¹,

Mattos²,

Pirovani³

et al. 2009

Genet. Mol. Res.

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“…98 Yeast that enter stationary phase through diauxic shift form two distinct populations, the heavier of which exhibits more of the characteristics of quiescent cells. 1 The heavier, quiescent population is unbudded, which indicates cell cycle arrest, and these heavier cells synchronously reenter the cell cycle when provided with nutrients.…”

Section: Introduction To Pathways That Regulate Metabolism: Autophagymentioning

confidence: 99%

Staying alive

et al. 2012

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Ensuring the continuity of life requires that individual cells and entire organisms can survive not only in ideal environments, but also in conditions of scarcity. When conditions are unfavorable for proliferation, many cells have the capacity to enter a nondividing state, sometimes for years, while retaining their ability to reenter the proliferative cell cycle. 1,2 This state of reversible cell cycle arrest, known as quiescence, is common and can be seen in stem cells, eggs and spores. Some quiescent cells are surprisingly hardy: they can survive long periods of nutrient starvation, cold temperatures and even desiccation. Entry into quiescence is often associated with dramatic changes in metabolism, defined as the uptake of nutrients and the use of these nutrients for the synthesis of macromolecules and energy. Indeed, proliferating and quiescent fibroblasts are expected to have very different metabolic requirements. While proliferating cells must devote much of their metabolic capacity to biosynthesis in order to create the material necessary to form a new cell, quiescent cells are relieved of this steep metabolic requirement. Many but not all quiescent cells respond by downregulating the synthesis of proteins *Correspondence to: Hilary Coller; Email: hcoller@princeton.edu Submitted: 02/22/12; Accepted: 02/27/12 http://dx.doi.org/10. 4161/cc.19879 Quiescence is a state of reversible cell cycle arrest that can grant protection against many environmental insults. in some systems, cellular quiescence is associated with a low metabolic state characterized by a decrease in glucose uptake and glycolysis, reduced translation rates and activation of autophagy as a means to provide nutrients for survival. For cells in multiple different quiescence model systems, including Saccharomyces cerevisiae, mammalian lymphocytes and hematopoietic stem cells, the Pi3Kinase/TOr signaling pathway helps to integrate information about nutrient availability with cell growth rates. Quiescence signals often inactivate the TOr kinase, resulting in reduced cell growth and biosynthesis. However, quiescence is not always associated with reduced metabolism; it is also possible to achieve a state of cellular quiescence in which glucose uptake, glycolysis and flux through central carbon metabolism are not reduced. in this review, we compare and contrast the metabolic changes that occur with quiescence in different model systems. 2 The signals for quiescence vary for different types of cells. Bacteria and yeast can enter stationary phase, a condition in which they cease cell growth and proliferation, in response to depletion of the glucose in their culture medium or in response to deprivation for a specific nutrient. In mammals, the proliferative state of individual cells is regulated by a host of factors that include not only the presence or absence of nutrients, but also cues from proliferative signaling molecules such as mitogens and situational cues. Quiescent T lymphocytes respond to stimulation of their T-cell receptor with the approp...

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“…These include the target of rapamycin (TOR) pathway (9), the protein kinase C-mitogen-activated protein (MAP) kinase pathway (34,44,55,65), the high-osmolarity glycerol-MAP kinase cascade (70), the Snf1 protein kinase pathway (61), and the protein kinase A-MAP kinase pathway (30,79,80). Additional signaling pathways that respond to changes in other environmental parameters may also feed in to the regulation of Msn2/4 and the general stress response.…”

mentioning

confidence: 99%

Dynamical Remodeling of the Transcriptome during Short-Term Anaerobiosis in Saccharomyces cerevisiae: Differential Response and Role of Msn2 and/or Msn4 and Other Factors in Galactose and Glucose Media

Lai¹,

Kosorukoff

Burke³

et al. 2005

Molecular and Cellular Biology

117

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In contrast to previous steady-state analyses of the O 2 -responsive transcriptome, here we examined the dynamics of the response to short-term anaerobiosis (2 generations) in both catabolite-repressed (glucose) and derepressed (galactose) cells, assessed the specific role that Msn2 and Msn4 play in mediating the response, and identified gene networks using a novel clustering approach. Upon shifting cells to anaerobic conditions in galactose medium, there was an acute (ϳ10 min) yet transient (<45 min) induction of Msn2-and/or Msn4-regulated genes associated with the remodeling of reserve energy and catabolic pathways during the switch from mixed respiro-fermentative to strictly fermentative growth. Concomitantly, MCB-and SCB-regulated networks associated with the G 1 /S transition of the cell cycle were transiently down-regulated along with rRNA processing genes containing PAC and RRPE motifs. Remarkably, none of these gene networks were differentially expressed when cells were shifted in glucose, suggesting that a metabolically derived signal arising from the abrupt cessation of respiration, rather than O 2 deprivation per se, elicits this "stress response." By ϳ0.2 generation of anaerobiosis in both media, more chronic, heme-dependent effects were observed, including the down-regulation of Hap1-regulated networks, derepression of Rox1-regulated networks, and activation of Upc2-regulated ones. Changes in these networks result in the functional remodeling of the cell wall, sterol and sphingolipid metabolism, and dissimilatory pathways required for long-term anaerobiosis. Overall, this study reveals that the acute withdrawal of oxygen can invoke a metabolic state-dependent "stress response" but that acclimatization to oxygen deprivation is a relatively slow process involving complex changes primarily in heme-regulated gene networks.

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Nutrient-induced signal transduction through the protein kinase A pathway and its role in the control of metabolism, stress resistance, and growth in yeast

Cited by 128 publications

References 43 publications

Carbon source-induced changes in the physiology of the cacao pathogen Moniliophthora perniciosa (Basidiomycetes) affect mycelial morphology and secretion of necrosis-inducing proteins

Carbon source-induced changes in the physiology of the cacao pathogen Moniliophthora perniciosa (Basidiomycetes) affect mycelial morphology and secretion of necrosis-inducing proteins

Staying alive

Dynamical Remodeling of the Transcriptome during Short-Term Anaerobiosis in Saccharomyces cerevisiae: Differential Response and Role of Msn2 and/or Msn4 and Other Factors in Galactose and Glucose Media

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