Nutrient sensing and metabolic changes after methionine deprivation in primary muscle cells of turbot ( Scophthalmus maximus L.)

Jiang, Haowen; Bian, Fuyun; Zhou, Huihui; Wang, Xuan; Wang, Kaidi; Mai, Kangsen; He, Gang

doi:10.1016/j.jnutbio.2017.08.015

Cited by 51 publications

(37 citation statements)

References 79 publications

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“…Moreover, IGF‐I binding to its receptor in the cell membrane activates the TOR protein via the PI3K/Akt pathway and regulates protein synthesis (Chen, Alberts, & Li, ). In the present study, fish fed relatively low Met diets (7.2–10.0 g/kg) showed lower mRNA levels of TOR, in agreement with other studies (Jiang et al, ; Rolland et al, ; Skiba‐Cassy et al, ). In addition, a positive relation ( r = +.815, p < .026, Table ) in the present study was observed between the mRNA levels of hepatic TOR and IGF‐I, indicating that feeding fish a Met‐deficient diet might suppress the TOR signalling pathway via IGF‐I leading to decreased cobia growth (Figure ).…”

Section: Discussionsupporting

confidence: 93%

“…Pyruvate kinase regulates the final rate‐limiting step of glycolysis and catalyses the dephosphorylation of phosphoenolpyruvate (PEP) to produce pyruvate and ATP (Chaneton & Gottlieb, ). In the present study, fish fed 7.2 g/kg dietary Met had the lowest expression of PK gene, which is similar to results in Met‐deprived primary cells in turbot (Jiang et al, ). In addition, 12.4 g/kg of dietary Met significantly improved the glycolysis capacity by enhancing PK mRNA levels, suggesting that the optimal dietary Met level could improve the conversion of glucose into glucose‐6‐phosphate and hepatic glucose utilization.…”

Section: Discussionsupporting

confidence: 90%

“…In the present study, fish fed a Met‐deficient diet had lower gene expression of hepatic SREBP1 compared with fish fed 12.4 g/kg Met, which was also accompanied by lower TOR, suggesting that the Met‐deficient diet suppressed SREBP1 via decreasing TOR in cobia (Figure ). A similar study performed in the primary muscle cells of turbot, showed that TOR activity was reduced while the expression level of SREBP1 was downregulated during Met deprivation (Jiang et al, ). In addition, increased TOR mRNA levels in fish fed 12.4 g/kg Met were accompanied by higher SREBP1, which indicates that SREBP1 might be mediated partly by increasing TOR expression during optimal dietary Met.…”

Section: Discussionmentioning

confidence: 82%

“…TOR signalling induces the expression of the transcription factor sterol regulatory element‐binding protein 1c (SREBP1c) (Düvel et al, ), which plays a key role in lipid metabolism by regulating the expression of genes that contribute to fatty acid synthesis (Shimano, ). TOR activity in primary muscle cells of turbot is reduced, and the expression level of SREBP1 is downregulated during Met deprivation (Jiang et al, ). In addition, Met in fish feed can regulate the activities and mRNA levels of enzymes involved in hepatic lipogenesis, such as hepatic SREBP1, fatty acid synthase (FAS) and ATP citrate lyase (Espe, Rathore, Du, Liaset, & El‐Mowafi, ; Rolland et al, ; Skiba‐Cassy, Geurden, Panserat, & Seiliez, ; Wang et al, ).…”

Section: Introductionmentioning

confidence: 99%

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Dietary methionine affects growth and the expression of key genes involved in hepatic lipogenesis and glucose metabolism in cobia (Rachycentron canadum)

Chi

Zhu

et al. 2019

Aquacult Nutr

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Juvenile cobia (Rachycentron canadum), a carnivorous fish, was chosen to examine the effects of methionine (Met) supplementation of low fishmeal diets on growth and the expression of key genes related to hepatic lipogenesis and glucose metabolism. Seven isonitrogenous and isolipidic diets were formulated to contain 7.2, 9.0, 10.0, 12.4, 14.1, 16.3 and 18.6 g/kg dietary Met levels. Healthy cobia (initial average weight ± SE = 9.79 ± 0.04 g) were randomly assigned into seven groups in triplicate with 40 individuals per sea cage for 8 weeks. The results showed that the weight gain rate (WGR) of fish increased as the dietary Met was increased up to 12.4 g/kg and then decreased. The expression of insulin‐like growth factor‐I (IGF‐I) and target of rapamycin (TOR) genes was inhibited by the Met‐deficient diets. The mRNA levels of fatty acid synthase (FAS) and sterol regulatory element‐binding protein‐1 (SREBP1) genes increased with increasing dietary Met until 12.4 g/kg after which the former remained constant, whereas the latter decreased from 12.4 to 18.6 g/kg Met. The mRNA levels of pyruvate kinase (PK) increased as the dietary Met levels increased, reaching a peak at 12.4 g/kg, and then decreased with further increases in Met. However, the mRNA levels of phosphoenolpyruvate carboxykinase (PEPCK) decreased as the dietary Met was increased up to 12.4 g/kg and then remained constant from 12.4 to 18.6 g/kg Met. In conclusion, based on the straight broken‐line analysis of WGR against dietary Met levels, the optimal dietary Met requirement for juvenile cobia was estimated to be 12.4 g/kg (26.9 g/kg dietary protein). A diet with the optimal level of Met induces fish growth and is associated with increased IGF‐I and TOR.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 82%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Dietary methionine affects growth and the expression of key genes involved in hepatic lipogenesis and glucose metabolism in cobia (Rachycentron canadum)

Chi

Zhu

et al. 2019

Aquacult Nutr

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“…The impact of nutrient sensing on food intake regulation and energy metabolism has been widely investigated in fish (e.g. Babaei et al, 2017; Comesana et al, 2018; Comesaña et al, 2018; Jiang et al, 2017; Li et al, 2017; Tian et al, 2018; Xu et al, 2016), and has been reviewed by Conde-Sieira and Soengas (2017). However, effects of nutritive and non-nutritive meals on gut motility and intestinal epithelium metabolism in fish remains largely unknown, except for a study in motility patterns in fed and starved sculpin ( Myoxocephalus scorpius ) (Brijs et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Nutrient sensing; transcriptomic response and regulation of gut motility in an agastric vertebrate

Lie

Etayo

et al. 2019

Preprint

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7 93 juveniles as a model. This fish does not have a stomach and the whole intestine was used for the 94 study. Differences in gene expression were compared between intestines administered cellulose 95 versus empty intestines in order to isolate the effect of mechanical sensing; and intestines 96 administered with either protein or lipid versus cellulose to compare effects of chemical sensing on 97 enterocyte metabolism. We also described postprandial motility patterns as responses to the 98 administration of the compounds in the ballan wrasse intestines. 99 2. Materials and Methods 100 2.1. Nutrient bolus 101 Six different diets, including intact lipid (IL), hydrolyzed lipid (HL), intact protein (IP), hydrolyzed 102 protein (HP), cellulose (CL) and plastic beads (PB) were prepared. Intact lipid was made by stirring a 103 mix of 80% (by volume) cod liver oil (Møllers Tran, containing omega-3-fatty acids and Vitamin D), 104 15% phosphate-buffered saline (PBS) pH = 8, and 5% Tween 20 (TWEEN® 20, P9416 Sigma Aldrich) to 105 obtain the same viscosity as the hydrolyzed lipid diet. To make 5 mL hydrolyzed lipid diet, we 106 incubated 4 mL cod liver oil with 3.5 mg lipase (Lipase from Pseudomonas cepacia -62309 Sigma Aldrich, 107 ≥30 U/mg) dissolved in 750 µL PBS (pH = 8) at 40 o C for 5 hours. The pH of the mix was maintained at 108 around 8 by titrating with 5 M NaOH during incubation. The mix was then incubated at 80 o C for 2 109 hours to deactivate the lipase before mixing with 250 µL Tween. 20. Five mL of intact protein diet was 110 prepared by combining 2 g casein with 4 mL 100 mM NH4HCO3 and 1 mL 1 mM HCl. Hydrolyzed 111 protein diet was prepared by incubating 5 mL intact protein diet with 43.7 mg trypsin (Trypsin Powder, 112 Porcine 1:250, 85450C, Sigma Aldrich) at 37 o C for 20 hours, followed by 80 o C for 2 hours for enzyme 113 deactivation. 5 mL of hydrolyzed protein diet was freshly mixed with 100 µL protease inhibitor 114 [Protease/Phosphatase Inhibitor Cocktail (100X) #5872, Cell Signaling Technology, Inc, The 115 Netherlands] before administration of the bolus into the intestine. Cellulose diet was created using 0.5 116 mg cellulose (Cellulose microcrystalline powder, 435236 Sigma Aldrich) in 1.5 mL H2O, adding a tiny 117 amount of Brillant Blue (Brilliant Blue R, B7920 Sigma-Aldrich) as a color maker. These five bolus types 118 were made and stored at -20 o C for use within a week. The diets were thawed and warmed up at 14 o C

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Replacing fishmeal with soybean meal affects survival, antioxidant capacity, intestinal microbiota, and mRNA expression of TOR and S6K1 in Macrobrachium rosenbergii

et al. 2021

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Nutrient sensing and metabolic changes after methionine deprivation in primary muscle cells of turbot ( Scophthalmus maximus L.)

Cited by 51 publications

References 79 publications

Dietary methionine affects growth and the expression of key genes involved in hepatic lipogenesis and glucose metabolism in cobia (Rachycentron canadum)

Dietary methionine affects growth and the expression of key genes involved in hepatic lipogenesis and glucose metabolism in cobia (Rachycentron canadum)

Nutrient sensing; transcriptomic response and regulation of gut motility in an agastric vertebrate

Replacing fishmeal with soybean meal affects survival, antioxidant capacity, intestinal microbiota, and mRNA expression of TOR and S6K1 in Macrobrachium rosenbergii

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