Nutrients in the fate of pluripotent stem cells

Lu, Vivian; Roy, Irena J.; Teitell, Michael A.

doi:10.1016/j.cmet.2021.09.013

Cited by 25 publications

(21 citation statements)

References 141 publications

(194 reference statements)

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“…There is growing, multidisciplinary appreciation for the role of nutrients in governing developmental cell fate decisions ( Lu et al., 2021 ). An increased understanding for how specific nutrients that are present in certain niches in vivo , and our ability to alter these nutrients in hPSC culture media, provides a valuable experimental tool to yield new insights into the regulation of early cell fate transitions, potentially within a developing human embryo.…”

Section: Expected Outcomesmentioning

confidence: 99%

Protocol for germ lineage differentiation of primed human pluripotent stem cells using chemically defined, nutrient-balanced media

Doan²,

Roy³

et al. 2022

STAR Protocols

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Section: Expected Outcomesmentioning

confidence: 99%

Protocol for germ lineage differentiation of primed human pluripotent stem cells using chemically defined, nutrient-balanced media

Doan²,

Roy³

et al. 2022

STAR Protocols

Self Cite

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Section: Introductionmentioning

confidence: 99%

“…Nutrients are normally crucial in SC physiology due to the ability of many nutrient-derived metabolites, released during the catabolic process, to induce chromatin reshaping, epigenetic modifications and gene expression modulation [ 20 ]. In this context, for example, glucose has been reported to act as an SC fate regulator, controlling key phases of embryo development [ 20 , 21 , 22 ]. Similarly, glycolysis and acetyl-CoA have been described to play an active role in the maintenance of pluripotency through induction of histone acetylation; accordingly, modifications of glycolysis or administration of acetyl-CoA precursors can inhibit differentiation and histone deacetylation [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Role of Diet in Stem and Cancer Stem Cells

Puca

Fedele

Rasio

et al. 2022

IJMS

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Diet and lifestyle factors greatly affect health and susceptibility to diseases, including cancer. Stem cells’ functions, including their ability to divide asymmetrically, set the rules for tissue homeostasis, contribute to health maintenance, and represent the entry point of cancer occurrence. Stem cell properties result from the complex integration of intrinsic, extrinsic, and systemic factors. In this context, diet-induced metabolic changes can have a profound impact on stem cell fate determination, lineage specification and differentiation. The purpose of this review is to provide a comprehensive description of the multiple “non-metabolic” effects of diet on stem cell functions, including little-known effects such as those on liquid-liquid phase separation and on non-random chromosome segregation (asymmetric division). A deep understanding of the specific dietetic requirements of normal and cancer stem cells may pave the way for the development of nutrition-based targeted therapeutic approaches to improve regenerative and anticancer therapies.

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“…During terminal differentiation, self-renewing stem cells must exit the cell cycle and progressively generate a progenitor cell population that differentiates into functional cell types (Liu et al, 2019; Pauklin and Vallier, 2013). As self-renewing stem cells intrinsically preserve proliferation and oppose differentiation programs, metabolic and nutrient signals play critical roles in driving the transition from self-renewal to differentiation (Arnold et al, 2022; Baksh et al, 2020; Chakrabarty and Chandel, 2021; Chakraborty et al, 2019; Lu et al, 2021; TeSlaa et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

A cell cycle-linked mechanism for the glutamine driven establishment of stem cell fate

Xiao

Meek

et al. 2022

Preprint

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The cell cycle offers a unique opportunity for stem cells to sample metabolic and signaling cues to establish cell fate. Molecular pathways that integrate and convey these signals to cell cycle machinery to license cell fate transitions and drive terminal differentiation remain unknown. Here, we describe a signaling role of mitochondrial glutamine metabolism in driving exit from cell cycle-linked self-renewal to generate differentiation competent progenitors. In proliferating stem cells, mitochondrial glutamine metabolism opposes the WDR5-linked self-renewal network via acetylation and nuclear translocation of its upstream regulator, PASK. Nuclear PASK disrupts the mitotic WDR5-anaphase-promoting complex (APC/C) interaction to drive exit from self-renewal. Consistent with these roles, loss of PASK or inhibition of glutamine metabolism preserves stemness in vitro and in vivo during muscle regeneration. Our results suggest a mechanism whereby the proliferative functions of glutamine metabolism are co-opted by stem cells to establish cell fate.

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Nutrients in the fate of pluripotent stem cells

Cited by 25 publications

References 141 publications

Protocol for germ lineage differentiation of primed human pluripotent stem cells using chemically defined, nutrient-balanced media

Protocol for germ lineage differentiation of primed human pluripotent stem cells using chemically defined, nutrient-balanced media

Role of Diet in Stem and Cancer Stem Cells

A cell cycle-linked mechanism for the glutamine driven establishment of stem cell fate

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