Nutritive value and persistence of different alfalfa cultivars, but very little information is available in the literature on mineral concentrations, especially on microelements. This study was carried out to determine crude protein and mineral contents in alfalfa cultivars from two different locations, Ankara and Konya provinces, in Central Anatolia during 2005 and 2007. The experiment was conducted using a randomized complete block design with three replications. Significant differences in many investigated traits were found between cultivars, years, locations and location x year, location x cultivar, year x cultivar, and location x year x cultivar interactions. A crude protein ratio and micro and macro element contents (K,