Nutrition and Growth of Several Blue-Green Algae

Kratz, William A.; Myers, Jack

doi:10.2307/2438564

Cited by 530 publications

(163 citation statements)

References 14 publications

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“…Generation time was determined according to Kratz and Myers (1955) and biochemical tests such as nitrate reduction, catalase, urease and methyl red were performed following the methods of Benson (1998).…”

Section: Isolation and Enumeration Of Diazotrophic Endophytic Bacteriamentioning

confidence: 99%

Endophytic colonization ofTypha australisby a plant growth-promoting bacteriumKlebsiella oxytocastrain GR-3

Jha

Kumar

2007

Journal of Applied Microbiology

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Aims: To isolate and characterize endophytic diazotrophic bacteria from a semi‐aquatic grass (Typha australis) which grows luxuriantly with no addition of any nitrogen source. Methods and Results: Ten endophytic diazotrophic bacteria from surface‐sterilized roots and culm of T. australis were isolated and screened for plant growth‐promoting activities employing standard methods. Based on the rate of nitrogenase activity, indole acetic acid (IAA) production and phosphate (P) solubilization, one root isolate namely GR‐3 was found to be the most efficient one. This isolate was identified as Klebsiella oxytoca on the basis of 16S rDNA sequence analysis. Amplification of nifH by polymerase chain reaction (PCR) and detection of dinitrogenase reductase by western blot confirmed the diazotrophic nature of GR‐3. It was tagged with gusA fused to a constitutive promoter and the resulting transconjugant was inoculated onto endophyte‐free rice variety Malviya dhan‐36 seedlings to express cross‐infection ability which resulted in a significant increase in root/shoot length and chlorophyll a content. Conclusions: Roots and culm of T. australis harbour several endophytic diazotrophic bacteria. One root isolate, identified as K. oxytoca GR‐3, seems to be an efficient plant growth‐promoting bacterium. Significance and Impact of the Study: Plant growth‐promoting properties of GR‐3 suggest that this promising isolate merits further investigations for potential application in agriculture.

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Section: Isolation and Enumeration Of Diazotrophic Endophytic Bacteriamentioning

confidence: 99%

Endophytic colonization ofTypha australisby a plant growth-promoting bacteriumKlebsiella oxytocastrain GR-3

Jha

Kumar

2007

Journal of Applied Microbiology

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“…Synechococcus PCC 6301 and PCC 7942 were cultured in AC medium (Kratz and Meyers, 1955) as modified by Shehata and Whitton (1982). Synechococcus PCC 7942 (R2-P1M8), a methionine auxotroph also lacking the 8 kb plasmid, was cultured in Allen's medium (Allen, 1968) supplemented with DLmethionine (30 jxg ml"') and streptomycin (5 pg ml"'').…”

Section: Cyanobacteriai Cuiture and Transformationmentioning

confidence: 99%

Isolation of a prokaryotic metallothionein locus and analysis of transcriptional control by trace metal ions

Huckle

Morby

Turner

et al. 1993

Molecular Microbiology

207

147

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In eukaryotes, metallothioneins (MTs) are involved in cellular responses to elevated concentrations of certain metal ions. We report the isolation and analysis of a prokaryotic MT locus from Synechococcus PCC 7942. The MT locus (smt) includes smtA, which encodes a class II MT, and a divergently transcribed gene, smtB. The sites of transcription initiation of both genes have been mapped and features within the smt operator-promoter region identified. Elevated concentrations of the ionic species of Cd, Co, Cr, Cu, Hg, Ni, Pb and Zn elicited an increase in the abundance of smtA transcripts. There was no detectable effect of elevated metal (Cd) on smtA transcript stability. Sequences upstream of smtA, fused to a promoterless lacZ gene, conferred metal-dependent beta-galactosidase activity in Synechococcus PCC 7942 (strain R2-PIM8). At maximum permissive concentrations, Zn was the most potent elicitor in vivo, followed by Cu and Cd with slight induction by Co and Ni. The deduced SmtB polypeptide has similarity to the ArsR and CadC proteins involved in resistance to arsenate/arsenite/antimonite and to Cd, contains a predicted helix-turn-helix DNA-binding motif and is shown to be a repressor of transcription from the smtA operator-promoter.

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“…Stream 1 received the lower incident intensity of 66,700 cal m-2 day-' (120 ft-c) provided by 100-w bulbs suspcndcd about 17 inches (43 cm 1,080,OOO cal m-" day-l given by Strickland ( 1960). Kratz and Meyer ( 1955) reported growth rates to increase rapidly when light was increased from 100 to 180 ft-c ( 1,076-1,940 ILIX) and to increase more slowly when light was further increased to 260 Et-c (2,800 lux ) . A report on Clzlorelh cultures by Myers (1946) g avc 35 ft-c ( 378 lux ) as the in tcnsity for the greatest photosynthetic capacity, probably referring to efficiencies, and 100 ft-c (1,076 lux) as the intensity for the greatest growth rate.…”

Section: Effects Of Study Conditionsmentioning

confidence: 98%

Primary Productivity and Energy Relationships in Artificial Streams1

Kevern

Ball

1965

Limnology & Oceanography

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The productivity of algal communities was investigated in two recirculating artificial streams. Emphasis was placed on a study of the effects of various factors on energy flow and on the characterization of artificial streams as a research tool. Productivity was measured by pH and carbon dioxide changes, upstream‐downstream oxygen changes, and the biomass of algae collected on artificial substrata. A temperature increase caused a small, but insignificant, increase in net productivity, while increased light intensity caused an increase in net productivity. A higher current velocity caused an increase in net productivity, significant as shown by riffle vs. pool communities, but not significant between a fast and a slow stream. A shorter, but more frequent photoperiod did not change the rate of production significantly. High chelate concentration caused a reduction in net productivity. Estimates of net productivity by the carbon dioxide and oxygen methods were in agreement for two out of five studies, while estimates by the biomass of algae on substrata were consistently lower than estimates by the gas methods. Net production varied from 2,815 to 5,565 cal m−2 day−1, resulting in efficiencies from 3.8 to 9.8%. Efficiencies of gross productivity varied from 6.4 to 16.1%.

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Nutrition and Growth of Several Blue-Green Algae

Cited by 530 publications

References 14 publications

Endophytic colonization ofTypha australisby a plant growth-promoting bacteriumKlebsiella oxytocastrain GR-3

Endophytic colonization ofTypha australisby a plant growth-promoting bacteriumKlebsiella oxytocastrain GR-3

Isolation of a prokaryotic metallothionein locus and analysis of transcriptional control by trace metal ions

Primary Productivity and Energy Relationships in Artificial Streams1

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