Nutrition, Growth, and Reproduction

Catabolism in Mollicutes

“…However, during aerobic growth only a small quantity (10%) of the glycerol metabolized is incorporated into cellular material (principally glycerides), and GP is oxidized to triose phosphate by a G P oxidase. This reaction is essentially irreversible, consumes molecular oxygen and produces 1 mol of H 2 0 2 per mol of GP (Rodwell & Mitchell, 1979). However, the in vitro growth characteristics of a G P oxidase-negative strain of M .…”

“…mycoides, glycerol is required for growth in defined medium (Rodwell & Mitchell, 1979) ed to L-a-glycerophosphate (GP), an essential precursor of lipid synthesis. However, during aerobic growth only a small quantity (10%) of the glycerol metabolized is incorporated into cellular material (principally glycerides), and GP is oxidized to triose phosphate by a G P oxidase.…”

Enzymological Features of Aromatic Amino Acid Biosynthesis Reflect the Phylogeny of Mycoplasmas

“…A biochemical explanation for the observation that nutritional independence for aromatic amino acids depends upon the presence of L-alanine in A. laidlawii (Rodwell & Mitchell, 1979) has not yet been made. L-Alanine did not activate any of the aromatic-pathway enzymes examined.…”

“…Several groups have reported that mycoplasmas appear to possess an intact pathway for the biosynthesis of aromatic amino acids (Rodwell & Mitchell, 1979;Smith, 1971), based upon the use of Acholeplusmu luidluwii, the least fastidious mycoplasma species. Thus, A. luidluwii did not require L-phenylalanine and L-tyrosine for growth (provided that L-alanine was supplied), and 14C-labelled shikimic acid was incorporated into protein.…”

“…Thus, A. luidluwii did not require L-phenylalanine and L-tyrosine for growth (provided that L-alanine was supplied), and 14C-labelled shikimic acid was incorporated into protein. Mycoplusma mycoides strain Y, for which completely defined media are available, requires both L-phenylalanine and L-tyrosine (Rodwell & Mitchell, 1979).…”