Nutrition of Animal Cells in Tissue Culture. X. Synthetic Medium No. 858,

Healy, George M.; Fisher, Dorothy C.; Parker, Raymond C.

doi:10.3181/00379727-89-21719

Cited by 90 publications

(7 citation statements)

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“…The standard culture medium was Medium 858 (Healy, Fisher & Parker, 1955) (Commonwealth Serum Laboratories, Melbourne) with added glucose (0-1 g%) and 20 % by volume of serum, and the culture technique used was as described previously (Heath & Smyth, 1970;Rickard & Bell, 1971). In some experiments the culture medium was altered either by using varying concentrations of rabbit serum (2-5-20 %) or by using 10 % by volume of various fractions of rabbit serum prepared by ultrafiltration (as described below) instead of the 20 % of normal rabbit serum.…”

Section: Cultivation Of T Pisiformis Oncospheres and Collection Of 'mentioning

confidence: 99%

Partial purification of antigens collected duringin vitrocultivation of the larval stages ofTaenia pisiformis

Rickard

Katiyar

1976

Parasitology

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SummaryLarvae of Taenia pisiformis were cultured in vitro in medium containing 2·5, 5 or 20% (v/v) of normal rabbit serum (NRS). Greatest development occurred in 20% NRS, and the potency of antigens collected in medium from each culture tested by intradermal (i/d) skin tests in infected rabbits paralleled the in vitro growth rate of larvae. ‘Culture’ antigens from 5% NRS stimulated good immunity in rabbits to a challenge infection with T. pisiformis eggs, although they were poorly reactive in skin tests.T. pisiformis larvae were also cultured in 10% (v/v) of nitrates of serum reduced to one-half of its volume by passage through 300 000 MW cut oif (XM300F) or 100000 MW cut off (XM100F) ultrafiltration membranes. Larvae cultured using XM300F had growth rates comparable with those cultured in 20% NRS, and the antigens released into the culture medium had equal potency in i/d tests and in stimulating protective immunity in rabbits. Larvae did not develop in XM100F orproduce skin-reactive or protective antigens.Crude ‘culture’ antigen from cultures in 20% NRS was separated into 4 fractions by nitration on Sephadex G200. All of these fractions gave i/d skin reactions in infected rabbits. Fraction 3 (F3) was the most active, but was shown by acrylamide gel electrophoresis and immunoelectrophoresis to be highly contaminated with rabbit serum proteins. F3 was separated into fractions on DEAE-Sephadex A50, and the third fraction from this was as active as the original culture medium in i/d skin tests, but had only 5% of the original protein concentration. Electrophoresis demonstrated few serum contaminants, and 2 indistinct protein bands that were not present in a similar fraction of NRS.Neither Sephadex G200 F3 nor DEAE-Sephadex F3 stimulated protective immunity in rabbits, suggesting that antigens stimulating immunity against the establishment of T. pisiformis in rabbits and those provoking cell-mediated immune reactions may be different.

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Section: Cultivation Of T Pisiformis Oncospheres and Collection Of 'mentioning

confidence: 99%

Partial purification of antigens collected duringin vitrocultivation of the larval stages ofTaenia pisiformis

Rickard

Katiyar

1976

Parasitology

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“…Human Embryonic Skin (H.E.S. ), a diploid fibroblast cell hne prepared according to the method of Ferguson and Wansbrough (1962), was grown in a mixture of 80 per cent medium 858 (Healy, Fisher and Parker, 1955) modified at C.S.L. (Commonwealth Serum Laboratories, 1964) and 20 per cent foetal calf serum with penicillin added to a final concentration of 100 units/ml., and streptomycin added to a final concentration of 100 ng./ml.…”

Section: Cellsmentioning

confidence: 99%

Growth of Diploid and Heteroploid Tissue Cultures in the Presence of Some Antibiotics

Tomkins¹,

Ferguson²

1965

Aust J Exp Biol Med

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Summary Several antibiotics were tested for their toxicity on three cell types—the heteroploid H.Ep.2 cell line, primary cynomolgus monkey kidney cells, and a diploid human embryonic skin line. The elimination of specific contaminants by particular antibiotics is discussed, and suitable concentrations of the antibiotics for use in tissue cultures are recommended. Kanamycin, neomycin, fungizone, chloramphenicol, viomycin and polymyxin B are suitable antibiotics for the elimination of the micro‐organisms most frequently encountered as contaminants of tissue cultures.

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“…bottles. The asbestos pad generally used in such a filter was replaced by two layers of washed rayon net (as used by Shaffer (1956) (Healy, Fisher & Parker, 1955) The explant8. Hens' eggs were incubated for 7 days, and then the hind limbs of the chick embryos were removed and placed in Tyrode solution; the femur and tibia were dissected out and washed in several changes of this solution.…”

Section: By Mary F Hay* From the Strangeways Research Laboratory Andmentioning

confidence: 99%

“…1118 has the same composition as No. 858 (Healy, Fisher & Parker, 1955) except that in No. 1118 the concentration of each amino acid has been doubled.…”

mentioning

confidence: 99%

The effect of growth hormone and insulin on limb‐bone rudiments of the embryonic chick cultivated in vitro

Hay¹

1958

The Journal of Physiology

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Nutrition of Animal Cells in Tissue Culture. X. Synthetic Medium No. 858,

Cited by 90 publications

References 0 publications

Partial purification of antigens collected duringin vitrocultivation of the larval stages ofTaenia pisiformis

Partial purification of antigens collected duringin vitrocultivation of the larval stages ofTaenia pisiformis

Growth of Diploid and Heteroploid Tissue Cultures in the Presence of Some Antibiotics

The effect of growth hormone and insulin on limb‐bone rudiments of the embryonic chick cultivated in vitro

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