The Random Amplification of Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) analyses were applied for studying 7 Egyptian populations of crab species. The genetic distance (GD) among close collection sites was small among location 1, 2, 3, 4, 6 and 7, but those between location 5 and 8 were much larger (0.666 and 0.833, respectively). These values between the populations of Lake Timsah, Bitter Lakes, Suez, Attaka, Al-Tur, Port said, Domietta, Rashid and Abo-Qir showed that, AL-Tur population was distantly related from both Lake Timsah, Bitter Lakes, Suez, Attaka, Port-Said, Domietta, Rashid and Abo-Qir populations.Average of similarity index (SI) values among the studied populations based on RAPD analysis were 0.646, 0.229, 0.32, 0.328, 0.222 and 0.338 in respective arrangement between each pair of the populations; S.T.z with E.