The objective of the present work was to investigate the influence of pollen sources over chemical characteristics of the Medjool date cultivar. The study was conducted under conditions of the Northwest part of Mexico, during the year 2016 cultivation season. The content of nutrients (protein, lipids, fiber and ash), sugars (total, reducing, non-reducing, sucrose, glucose and fructose), and most abundant minerals were evaluated. Three bunches of each of the four selected female palms Medjool cultivar were pollinated with Deglet Noor, Khadrawy, Medjool and Zahidi cultivars, and their fruit were used for the analyses. Results show low concentrations of lipids and ash (0.68-0.80% and 2.49-2.82%, g/100 g dry weight basis), while protein and crude fiber were in the range of 2.92-3.35% and 6.86-7.29%, respectively. In addition, a high content of total sugars (77.78-70.04%) were found. The predominant mineral was potassium with a range of 794.30 (Khadrawy)-869.93 mg/100 g dry weight basis (Deglet Noor), followed by magnesium with 136.72 (Medjool)-150.10 mg/100 g dry weight basis (Khadrawy). An analysis of total phenols revealed that the Khadrawy cultivar had the highest content, with 5.30 (mg GAE/100 g) and the lowest was for Zahidi with 3.82 (mg GAE/100 g). The Khadrawy cultivar had the highest effects on the chemical parameters of the four evaluated treatments. Therefore, pollen source has a significant effect on the chemical properties of the fruit from the Medjool cultivar.