Nutritional profile of High Fat Simple Carbohydrate Diet used to induce metabolic syndrome in C57BL/6J mice

D’Souza, Serena; Bindu, B. Sri Charan; Ali, Majdeddin Mohammed; Tisha, Alex; Deepthi, K.; Siona, Silveira; Santy, Fernandes; Abraham, Ambily

doi:10.1016/j.jnim.2015.12.334

Cited by 5 publications

(3 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our study, the insert insulin of group T had reduced values as compared to mice of group C, indicating decreased pancreatic b-cell function. This is further supported by histopathological observations in the pancreas of HFSC-fed C57BL/6J mice in our previous study [16].…”

Section: Prace Oryginalnesupporting

confidence: 87%

See 1 more Smart Citation

Insulinooporność poprzedza nietolerancję glukozy i hiperleptynemię u myszy C57BL/6J otrzymujących karmę wysokotłuszczową i zawierającą cukry proste

Bn¹,

D’Souza

Abraham³

2016

Endokrynologia Polska

View full text Add to dashboard Cite

Introduction: Very few systematic studies are done during the onset and progression of metabolic syndrome in suitable animal models. In this paper we present the effect of High-Fat Simple Carbohydrate (HFSC) feed on the metabolic hormones in C57BL/6J mice to understand the sequence of events leading to impairment of glucose homeostasis. Material and methods: One-month-old male C57BL/6J mice were fed with control (C group) and HFSC (T group) feed (n = 30 each) respectively for five months. The glucose tolerance was studied by Oral Glucose Tolerance Test (OGTT) whereas serum insulin and leptin were quantified using ELISA kits, and serum cortisol was quantified using CLIA kits. Results: Insulin resistance index and HOMA-IR levels were higher in the mice of group T as compared to age-matched mice of group C within one month and significantly higher after and five months of feeding. The total area under the glucose tolerance test curve (AUC) and the insulin curve (AUC ins) was found to significantly increase in the mice of T group as compared to the mice of C group as early as two months of feeding and was elevated after 5 months post feeding. Comparison of the Matsuda index revealed that pancreatic beta cell function was significantly lower in mice of T group as compared to mice of C group by five months of feeding. Leptin levels fluctuated during the 1 st -4 th month and by the 5 th month significant hyperleptinaemia was detected. There was no significant change in cortisol levels in mice of group T as compared to mice of group C after five months of feeding. Conclusions: HFSC feed induces insulin resistance by the first month and progressively impairs glucose tolerance, resulting in hyperleptinaemia by the fifth month in male C57BL/6J mice. (Endokrynol Pol 2016; 67 (6): 592-598)

show abstract

Section: Prace Oryginalnesupporting

confidence: 87%

“…Total energy content from control feed was 3582.369 Kcal/ kg and from HFSC feed was 5064.581 Kcal/kg [16]. The mice were provided with fresh food and water, both ad libitum, daily.…”

Section: Animals Usedmentioning

confidence: 99%

Insulinooporność poprzedza nietolerancję glukozy i hiperleptynemię u myszy C57BL/6J otrzymujących karmę wysokotłuszczową i zawierającą cukry proste

Bn¹,

D’Souza

Abraham³

2016

Endokrynologia Polska

View full text Add to dashboard Cite

show abstract

“…While no significant differences were noted between other parameters across quintiles, this Experiment 4 stratification of females into RBG quintiles substantiates the genetic permissiveness that occurs early in this T2DM model, first noted in Experiment 1. Compared to human epidemiological data involving diet x genetic variation in T2DM, the Nile rat is more like humans and less like mice and conventional rats, as humans and Nile rats are CHO-sensitive while mice and rats are not [10,11,13,23,70,71].…”

Section: Resultsmentioning

confidence: 99%

Genetic Permissiveness and Dietary Glycemic Load Interact to Predict Type-II Diabetes in the Nile rat (Arvicanthis niloticus)

2019

View full text Add to dashboard Cite

Objective: The Nile rat (Arvicanthis niloticus) is a superior model for Type-II Diabetes Mellitus (T2DM) induced by diets with a high glycemic index (GI) and glycemic load (GLoad). To better define the age and gender attributes of diabetes in early stages of progression, weanling rats were fed a high carbohydrate (hiCHO) diet for between 2 to 10 weeks. Methods. Data from four experiments compared two diabetogenic semipurified diets (Diet 133 (60:20:20, as % energy from CHO, fat, protein with a high glycemic load (GLoad) of 224 per 2000 kcal) versus Diets 73MBS or 73MB (70:10:20 with or without sucrose and higher GLoads of 259 or 295, respectively). An epidemiological technique was used to stratify the diabetes into quintiles of blood glucose (Q1 to Q5), after 2-10 weeks of dietary induction in 654 rats. The related metagenetic physiological growth and metabolic outcomes were related to the degree of diabetes based on fasting blood glucose (FBG), random blood glucose (RBG), and oral glucose tolerance test (OGTT) at 30 minutes and 60 minutes. Results. Experiment 1 (Diet 73MBS) demonstrated that the diabetes begins aggressively in weanlings during the first 2 weeks of a hiCHO challenge, linking genetic permissiveness to diabetes susceptibility or resistance from an early age. In Experiment 2, ninety male Nile rats fed Diet 133 (60:20:20) for 10 weeks identified two quintiles of resistant rats (Q1,Q2) that lowered their RBG between 6 weeks and 10 weeks on diet, whereas Q3-Q5 became progressively more diabetic, suggesting an ongoing struggle for control over glucose metabolism, which either stabilized or not, depending on genetic permissiveness. Experiment 3 (32 males fed 70:10:20) and Experiment 4 (30 females fed 60:20:20) lasted 8 weeks and 3 weeks respectively, for gender and time comparisons. The most telling link between a quintile rank and diabetes risk was telegraphed by energy intake (kcal/day) that established the cumulative GLoad per rat for the entire trial, which was apparent from the first week of feeding. This genetic permissiveness associated with hyperphagia across quintiles was maintained throughout the study and was mirrored in body weight gain without appreciable differences in feed efficiency. This suggests that appetite and greater growth rate linked to a fiber-free high GLoad diet were the dominant factors driving the diabetes. Male rats fed the highest GLoad diet (Diet 73MB 70:10:20, GLoad 295 per 2000 kcal for 8 weeks in Experiment 3], ate more calories and developed diabetes even more aggressively, again emphasizing the Cumulative GLoad as a primary stressor for expressing the genetic permissiveness underlying the diabetes. Conclusion: Thus, the Nile rat model, unlike other rodents but similar to humans, represents a superior model for high GLoad, low-fiber diets that induce diabetes from an early age in a manner similar to the dietary paradigm underlying T2DM in humans, most likely originating in childhood.

show abstract

Nutrition and toxicity properties of fried grasshopper (Oxya chinensis): The effect of air frying temperature and times of cooking

Iwansyah,

Latifatunnajib,

Kumalasari

et al. 2024

International Conference on Organic and Applied Chemistry (Icoac) 2022

View full text Add to dashboard Cite

Nutritional profile of High Fat Simple Carbohydrate Diet used to induce metabolic syndrome in C57BL/6J mice

Cited by 5 publications

References 47 publications

Insulinooporność poprzedza nietolerancję glukozy i hiperleptynemię u myszy C57BL/6J otrzymujących karmę wysokotłuszczową i zawierającą cukry proste

Insulinooporność poprzedza nietolerancję glukozy i hiperleptynemię u myszy C57BL/6J otrzymujących karmę wysokotłuszczową i zawierającą cukry proste

Genetic Permissiveness and Dietary Glycemic Load Interact to Predict Type-II Diabetes in the Nile rat (Arvicanthis niloticus)

Nutrition and toxicity properties of fried grasshopper (Oxya chinensis): The effect of air frying temperature and times of cooking

Contact Info

Product

Resources

About